@article{ea13aace8c334e92acbdfdb68578911c,
title = "Analytical method for the determination of sphinganine and sphingosine in serum as a potential biomarker for fumonisin exposure",
abstract = "The toxins produced by Fusarium moniliforme, which include fumonisins, are possible human carcinogens. Fumonisins are inhibitors of de novo sphingolipid biosynthesis. Alterations of the ratio of sphinganine (Sa) to sphingosine (So) in urine and serum has been proposed as a possible biomarker of exposure to this toxin. A new method was developed for their analysis in tissues and urine. This work describes the further adaptation of the method to the analysis of Sa and So in serum and its validation in sera of untreated and fumonisin B1 (FB1) treated rats and mice. No significant differences in the Sa/So ratios were observed in the FB1 treated rats. In mice, the increase was only of marginal statistical significance. Determination of Sa/So ratios in human sera could readily be made in small volumes (from 0.3 to 0.5 ml) of serum. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Biomarker, Fumonisins, Sphinganine, Sphingosine",
author = "M. Castegnaro and L. Garren and D. Galendo and Gelderblom, {W. C.A.} and Chelule, {Paul Kiprono} and Dutton, {M. F.} and Wild, {C. P.}",
note = "Funding Information: The authors wish to thank Prof. J.D. Miller for providing the fumonisin B 1 necessary for this investigation and Dr. A.H. Merrill for the gift of C20 Sa. We also thank the {\textquoteleft}Centre de Transfusion Sanguine de Lyon{\textquoteright} and Dr. A. Hafferjee and V.S. Naidu (Durban hospital, South Africa) for providing us with randomly collected human blood samples and Mr. Christophe Martire and Ms. St{\'e}phanie Riouffreyt (Trainees from the {\textquoteleft}Institut Universitaire Professionnel, Lyon{\textquoteright}), for their technical assistance. C.P. Wild acknowledges the support of National Institute of Environmental Health Sciences grant No ES06052 in performing this work; M. Castegnaro, support from the French Ministries of {\textquoteleft}Affaires Etrang{\`e}res{\textquoteright} and {\textquoteleft}Education Nationale, Recherche et Technologie{\textquoteright}; M. Dutton, Foundation for Research and Development for funding for UN group and (Maastricht University Center for International Cooperation in the Development of Education and; Paul Chelule, Moi University for a bursary. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",
year = "1998",
month = dec,
day = "11",
doi = "10.1016/S0378-4347(98)00446-0",
language = "English",
volume = "720",
pages = "15--24",
journal = "Journal of Chromatography - Biomedical Applications",
issn = "1572-6495",
publisher = "Elsevier BV",
number = "1-2",
}