Antiplasmodial activity of the n-Hexane extract from pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.

Ozadheoghene Eriarie Afieroho; Xavier Siwe Noundou; Chiazor P. Onyia; Osamuyi H. Festus; Elizabeth C. Chukwu; Olutayo M. Adedokun; Michelle Isaacs; Heinrich C. Hoppe; Rui W.M. Krause; Kio A. Abo

doi:10.4274/tjps.18894

Antiplasmodial activity of the n-Hexane extract from pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.

Ozadheoghene Eriarie Afieroho^*, Xavier Siwe Noundou, Chiazor P. Onyia, Osamuyi H. Festus, Elizabeth C. Chukwu, Olutayo M. Adedokun, Michelle Isaacs, Heinrich C. Hoppe, Rui W.M. Krause, Kio A. Abo

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Objectives: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC ₅₀ =0.016 μg/mL) > n-hexane extract (IC ₅₀ =25.18 μg/mL). It also showed mild cytotoxicity (IC ₅₀ >100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC ₅₀ =0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.

Original language	English
Pages (from-to)	37-42
Number of pages	6
Journal	Turkish Journal of Pharmaceutical Sciences
Volume	16
Issue number	1
DOIs	https://doi.org/10.4274/tjps.18894
Publication status	Published - Mar 2019
Externally published	Yes

Keywords

Cytotoxicity
Ergosterol
Malaria
Nutraceuticals
Pleurotus ostreatus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4274/tjps.18894

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title = "Antiplasmodial activity of the n-Hexane extract from pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.",

abstract = " Objectives: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC 50 =0.016 μg/mL) > n-hexane extract (IC 50 =25.18 μg/mL). It also showed mild cytotoxicity (IC 50 >100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC 50 =0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.",

keywords = "Cytotoxicity, Ergosterol, Malaria, Nutraceuticals, Pleurotus ostreatus",

author = "Afieroho, {Ozadheoghene Eriarie} and Noundou, {Xavier Siwe} and Onyia, {Chiazor P.} and Festus, {Osamuyi H.} and Chukwu, {Elizabeth C.} and Adedokun, {Olutayo M.} and Michelle Isaacs and Hoppe, {Heinrich C.} and Krause, {Rui W.M.} and Abo, {Kio A.}",

note = "Publisher Copyright: {\textcopyright} Turk J Pharm Sci, Published by Galenos Publishing House.",

year = "2019",

month = mar,

doi = "10.4274/tjps.18894",

language = "English",

volume = "16",

pages = "37--42",

journal = "Turkish Journal of Pharmaceutical Sciences",

issn = "1304-530X",

publisher = "Turkish Pharmacists Association",

number = "1",

}

TY - JOUR

T1 - Antiplasmodial activity of the n-Hexane extract from pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.

AU - Afieroho, Ozadheoghene Eriarie

AU - Noundou, Xavier Siwe

AU - Onyia, Chiazor P.

AU - Festus, Osamuyi H.

AU - Chukwu, Elizabeth C.

AU - Adedokun, Olutayo M.

AU - Isaacs, Michelle

AU - Hoppe, Heinrich C.

AU - Krause, Rui W.M.

AU - Abo, Kio A.

N1 - Publisher Copyright: © Turk J Pharm Sci, Published by Galenos Publishing House.

PY - 2019/3

Y1 - 2019/3

N2 - Objectives: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC 50 =0.016 μg/mL) > n-hexane extract (IC 50 =25.18 μg/mL). It also showed mild cytotoxicity (IC 50 >100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC 50 =0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.

AB - Objectives: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC 50 =0.016 μg/mL) > n-hexane extract (IC 50 =25.18 μg/mL). It also showed mild cytotoxicity (IC 50 >100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC 50 =0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.

KW - Cytotoxicity

KW - Ergosterol

KW - Malaria

KW - Nutraceuticals

KW - Pleurotus ostreatus

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DO - 10.4274/tjps.18894

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SN - 1304-530X

VL - 16

SP - 37

EP - 42

JO - Turkish Journal of Pharmaceutical Sciences

JF - Turkish Journal of Pharmaceutical Sciences

IS - 1

ER -

Antiplasmodial activity of the n-Hexane extract from pleurotus ostreatus (Jacq. ex. Fr) P. Kumm.

Abstract

Keywords

UN SDGs

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