TY - JOUR
T1 - Characterisation of rotavirus G9 strains isolated in the UK between 1995 and 1998
AU - Iturriza-Gómara, Miren
AU - Cubitt, David
AU - Steele, Duncan
AU - Green, Jonathan
AU - Brown, David
AU - Kang, Gagandeep
AU - Desselberger, Ulrich
AU - Gray, Jim
PY - 2000
Y1 - 2000
N2 - G9P[6] and G9P[8] rotavirus strains were identified during 1995/96 through the molecular epidemiological surveillance of rotavirus strains circulating in the UK between 1995 and 1998. An increase in the incidence and spread of sporadic infections with rotavirus genotype G9P[8] across the UK was detected in the two following seasons. Partial sequencing of the VP7 gene showed that all the UK strains shared a high degree of homology and were related very closely to G9 strains from the US and from symptomatic infections in India (≥96% homology). The UK strains were related more distantly to the apathogenic Indian strain 116E (85-87.8% homology). Phylogenetic analysis revealed clustering of the UK strains into 3 different lineages (I to III) and into two sub-lineages within lineage I. There were correlations between VP7 sequence clustering, the P type and the geographical origin of the G9 strains. Partial sequencing of the VP4 gene showed high degree of homology (>98%) among all the P[6] strains, and the sequences obtained from the P[8] strains clustered into 2 of the 3 global lineages described for P[8] strains associated with other G types. These data suggest that G9 strains may be a recent importation into the UK, and that G9P[8] strains may have emerged through reassortment in humans between G9P[6] strains introduced recently and the more prevalent cocirculating G1, G3 and G4 strains that normally carry VP4 genes of P[8] type. (C) 2000 Wiley-Liss, Inc.
AB - G9P[6] and G9P[8] rotavirus strains were identified during 1995/96 through the molecular epidemiological surveillance of rotavirus strains circulating in the UK between 1995 and 1998. An increase in the incidence and spread of sporadic infections with rotavirus genotype G9P[8] across the UK was detected in the two following seasons. Partial sequencing of the VP7 gene showed that all the UK strains shared a high degree of homology and were related very closely to G9 strains from the US and from symptomatic infections in India (≥96% homology). The UK strains were related more distantly to the apathogenic Indian strain 116E (85-87.8% homology). Phylogenetic analysis revealed clustering of the UK strains into 3 different lineages (I to III) and into two sub-lineages within lineage I. There were correlations between VP7 sequence clustering, the P type and the geographical origin of the G9 strains. Partial sequencing of the VP4 gene showed high degree of homology (>98%) among all the P[6] strains, and the sequences obtained from the P[8] strains clustered into 2 of the 3 global lineages described for P[8] strains associated with other G types. These data suggest that G9 strains may be a recent importation into the UK, and that G9P[8] strains may have emerged through reassortment in humans between G9P[6] strains introduced recently and the more prevalent cocirculating G1, G3 and G4 strains that normally carry VP4 genes of P[8] type. (C) 2000 Wiley-Liss, Inc.
KW - Genotype
KW - Phylogenetic analysis
KW - Rotavirus
KW - VP4
KW - VP7
UR - http://www.scopus.com/inward/record.url?scp=0343526337&partnerID=8YFLogxK
U2 - 10.1002/1096-9071(200008)61:4<510::AID-JMV15>3.0.CO;2-Q
DO - 10.1002/1096-9071(200008)61:4<510::AID-JMV15>3.0.CO;2-Q
M3 - Article
C2 - 10897071
AN - SCOPUS:0343526337
SN - 0146-6615
VL - 61
SP - 510
EP - 517
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 4
ER -