TY - JOUR
T1 - Characterization of human rotavirus strains from children with diarrhea in nairobi and kisumu, kenya, between 2000 and 2002
AU - Nyangao, James
AU - Page, Nicola
AU - Esona, Mathew
AU - Peenze, Ina
AU - Gatheru, Zipporah
AU - Tukei, Peter
AU - Steele, A. Duncan
N1 - Funding Information:
Financial support: Kenyan Medical Research Institute, South African Medical Research Council, World Health Organization (grant IVB27/181/113 to J.N. to support visit to South Africa), Poliomyelitis Research Foundation, Sandringham.
Funding Information:
Supplement sponsorship: This article is part of a supplement entitled “Rotavirus Infection In Africa: Epidemiology, Burden of Disease, and Strain Diversity,” which was prepared as a project of the Rotavirus Vaccine Program, a partnership among PATH, the World Health Organization, and the US Centers for Disease Control and Prevention, and was funded in full or in part by the GAVI Alliance.
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Rotavirus infection is a major cause of diarrheal illness and hospitalization in children <5 years old in Kenya and has been described in various settings and locations across the country and for different time points. In this study, we expand on the molecular characterization of rotavirus strains collected in Nairobi and Kisumu, Kenya, between 2000 and 2002. Rotavirus strains were typed by reverse-transcription polymerase chain reaction and characterized using VP6 monoclonal antibodies and RNA electrophoresis of the viral genome. A large proportion of specimens could not be genotyped; 41% did not produce a G type result, and 43% did not produce a P type result. Of the strains that could be genotyped, G1P[8] strains were predominant, followed by G2P[4] strains. In addition, G8 and G9 strains were seen in similar proportions Interestingly, the G and P combinations were more diverse among G8 and G9 rotavirus strains, suggesting the recent introduction of these strains into the human population. These observations are a link between the occasional observation of G8 and G9 strains at the turn of the century and the high predominance of G9P[8] strains observed in Kenya in 2005.
AB - Rotavirus infection is a major cause of diarrheal illness and hospitalization in children <5 years old in Kenya and has been described in various settings and locations across the country and for different time points. In this study, we expand on the molecular characterization of rotavirus strains collected in Nairobi and Kisumu, Kenya, between 2000 and 2002. Rotavirus strains were typed by reverse-transcription polymerase chain reaction and characterized using VP6 monoclonal antibodies and RNA electrophoresis of the viral genome. A large proportion of specimens could not be genotyped; 41% did not produce a G type result, and 43% did not produce a P type result. Of the strains that could be genotyped, G1P[8] strains were predominant, followed by G2P[4] strains. In addition, G8 and G9 strains were seen in similar proportions Interestingly, the G and P combinations were more diverse among G8 and G9 rotavirus strains, suggesting the recent introduction of these strains into the human population. These observations are a link between the occasional observation of G8 and G9 strains at the turn of the century and the high predominance of G9P[8] strains observed in Kenya in 2005.
UR - http://www.scopus.com/inward/record.url?scp=77955685127&partnerID=8YFLogxK
U2 - 10.1086/653564
DO - 10.1086/653564
M3 - Article
C2 - 20684701
AN - SCOPUS:77955685127
SN - 0022-1899
VL - 202
SP - S187-S192
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - SUPPL. 1
ER -