Detection of group A rotavirus strains circulating in calves in Tunisia

I. Fodha; A. Boumaiza; A. Chouikha; J. Dewar; G. Armah; A. Geyer; A. Trabelsi; A. D. Steele

doi:10.1111/j.1439-0450.2004.00810.x

Detection of group A rotavirus strains circulating in calves in Tunisia

I. Fodha^*, A. Boumaiza, A. Chouikha, J. Dewar, G. Armah, A. Geyer, A. Trabelsi, A. D. Steele

^*Corresponding author for this work

Virological Pathology

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Faecal samples were collected from 89 dairy calves to determine the prevalence of rotavirus infection in Tunisia and the genomic diversity of bovine rotavirus strains. After screening of all faecal samples by enzyme-linked immunosorbent assay, rotavirus strains were analysed by RNA polyacrylamide gel electrophoresis and characterized antigenically by monoclonal antibodies to the VP6 subgroup. The VP7 genotype was determined by nested RT-PCR. Of the 89 calves tested, 27 (30%) were positive for rotavirus antigen. Four different long electrophoretypes were identified. All VP6 typeable strains carried the subgroup I specificity. G8 genotype was the most prevalent, but G6 and mixed strains G(6 + 8) were also detected.

Original language	English
Pages (from-to)	49-50
Number of pages	2
Journal	Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health
Volume	52
Issue number	1
DOIs	https://doi.org/10.1111/j.1439-0450.2004.00810.x
Publication status	Published - Feb 2005

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abstract = "Faecal samples were collected from 89 dairy calves to determine the prevalence of rotavirus infection in Tunisia and the genomic diversity of bovine rotavirus strains. After screening of all faecal samples by enzyme-linked immunosorbent assay, rotavirus strains were analysed by RNA polyacrylamide gel electrophoresis and characterized antigenically by monoclonal antibodies to the VP6 subgroup. The VP7 genotype was determined by nested RT-PCR. Of the 89 calves tested, 27 (30%) were positive for rotavirus antigen. Four different long electrophoretypes were identified. All VP6 typeable strains carried the subgroup I specificity. G8 genotype was the most prevalent, but G6 and mixed strains G(6 + 8) were also detected.",

author = "I. Fodha and A. Boumaiza and A. Chouikha and J. Dewar and G. Armah and A. Geyer and A. Trabelsi and Steele, {A. D.}",

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T1 - Detection of group A rotavirus strains circulating in calves in Tunisia

AU - Fodha, I.

AU - Boumaiza, A.

AU - Chouikha, A.

AU - Dewar, J.

AU - Armah, G.

AU - Geyer, A.

AU - Trabelsi, A.

AU - Steele, A. D.

PY - 2005/2

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AB - Faecal samples were collected from 89 dairy calves to determine the prevalence of rotavirus infection in Tunisia and the genomic diversity of bovine rotavirus strains. After screening of all faecal samples by enzyme-linked immunosorbent assay, rotavirus strains were analysed by RNA polyacrylamide gel electrophoresis and characterized antigenically by monoclonal antibodies to the VP6 subgroup. The VP7 genotype was determined by nested RT-PCR. Of the 89 calves tested, 27 (30%) were positive for rotavirus antigen. Four different long electrophoretypes were identified. All VP6 typeable strains carried the subgroup I specificity. G8 genotype was the most prevalent, but G6 and mixed strains G(6 + 8) were also detected.

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Detection of group A rotavirus strains circulating in calves in Tunisia

Abstract

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