Detection of qnr genes and gyrA mutation to quinolone phenotypic resistance of UTI pathogens in Bangladesh and the implications

Tanjum Ara Haque, Umme Laila Urmi, Abul Bashar Mir Md Khademul Islam, Bayasrin Ara, Shamsun Nahar, Abu Syed Md Mosaddek, Halyna Lugova, Santosh Kumar, Dilshad Jahan, Nor Azlina A. Rahman, Mainul Haque, Salequl Islam*, Brian Godman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Plasmid-mediated quinolone-resistant (PMQR) genes and mutations within the quinolone resistancedetermining regions (QRDRs) resulted in the advent of quinolone-resistant pathogenic microbes. This research was designed to assess the roles of three PMQR genes, qnrA, qnrB, and qnrS, and any mutation in the gyrA gene in the QRDR as a process of quinolone/fluoroquinolone resistance to urinary tract infection (UTI) bacteria in Bangladesh to guide future management of UTIs. Methods: Pathogens from UTIs were isolated and identified, and their phenotype antibiotic susceptibilities were tested for lomefloxacin, ofloxacin, ciprofloxacin, and nalidixic acid. Polymerase chain reaction (PCR) detected the qnrA, qnrB, and qnrS genes. PCR and sequencing were performed to evaluate any mutation within the QRDRs of the gyrA gene. Results: Of 100 UTI bacteria, phenotypic resistance was observed in 95.0%, 89.0%, 83.0%, and 71.0% against lomefloxacin, nalidixic acid, ofloxacin, and ciprofloxacin, respectively. PMQR genes qnrS, qnrA, and qnrB genes were found in 54.0%, 1.0%, and 4.0% of isolates, respectively. Sequencing the gyrA gene revealed single mutation (Ser-83 to Leu) and double mutations (Ser-83 to Leu and Asp-87 to Asn). PMQR genes showed a statistically nonsignificant association with phenotypic resistance. Conclusions: This study confirms the presence of QRDR mutations that were independent of PMQR genes. Consequently, high resistance against quinolones among uropathogens is evident, and their future use needs to be moderated.

Original languageEnglish
Pages (from-to)185-198
Number of pages14
JournalJournal of Applied Pharmaceutical Science
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 2022

Keywords

  • Bangladesh
  • PMQR
  • qnr
  • QRDR
  • Quinolone
  • UTI

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