Determination of the G and P types of previously nontypeable rotavirus strains from the african rotavirus network, 1996-2004: Identification of unusual G types

Mathew Dioh Esona, Duncan Steele, Tara Kerin, George Armah, Ina Peenze, Annelise Geyer, Nicola Page, James Nyangao, Veronique Akran Agbaya, Abdelhalim Trabelsi, Bizuneh Tsion, Maryam Aminu, Theresia Sebunya, John Dewar, Roger Glass, Jon Gentsch

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.

Original languageEnglish
Pages (from-to)S49-S54
JournalJournal of Infectious Diseases
Volume202
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 1 Sept 2010
Externally publishedYes

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