TY - JOUR
T1 - Determination of the G and P types of previously nontypeable rotavirus strains from the african rotavirus network, 1996-2004
T2 - Identification of unusual G types
AU - Esona, Mathew Dioh
AU - Steele, Duncan
AU - Kerin, Tara
AU - Armah, George
AU - Peenze, Ina
AU - Geyer, Annelise
AU - Page, Nicola
AU - Nyangao, James
AU - Agbaya, Veronique Akran
AU - Trabelsi, Abdelhalim
AU - Tsion, Bizuneh
AU - Aminu, Maryam
AU - Sebunya, Theresia
AU - Dewar, John
AU - Glass, Roger
AU - Gentsch, Jon
PY - 2010/9/1
Y1 - 2010/9/1
N2 - A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.
AB - A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.
UR - http://www.scopus.com/inward/record.url?scp=77955706498&partnerID=8YFLogxK
U2 - 10.1086/653552
DO - 10.1086/653552
M3 - Article
C2 - 20684717
AN - SCOPUS:77955706498
SN - 0022-1899
VL - 202
SP - S49-S54
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - SUPPL. 1
ER -