TY - JOUR
T1 - Effect of Cinnamomum cassia Stem-Bark Extracts on the Synthesis and Secretion of Insulin in RIN-m5F Cells
AU - Kgopa, Ananias Hodi
AU - Makhubela, Sebolaishi Doris
AU - Mogale, Motetelo Alfred
AU - Shai, Leshweni Jerry
N1 - Funding Information:
The authors are thankful to the Research Development Grant, Sefako Makgatho Health Sciences University (Department of Biochemistry) for funding of this research and Director of the Intituitional Planning and Development, Prof BB Marvey for the financial support in purchasing cell culture equipments. Authors are also grateful to Dr OO Olaokun for donating RIN-m5F cells as well as Dr Mapula Razwinani in helping with the qPCR.
Funding Information:
The Research Development Grant of the Sefako Makgatho Health Sciences University.
Publisher Copyright:
© 2022 Ananias Hodi Kgopa, Sebolaishi Doris Makhubela, Motetelo Alfred Mogale and Leshweni Jerry Shai.
PY - 2022/4/5
Y1 - 2022/4/5
N2 - Cinnamomum cassia (C. cassia) crude stem-bark extracts possess some hypoglycemic properties through inhibiting the uptake of glucose by the intestines and/or enhancing insulin sensitivity. To further investigate the mechanism through which the hypoglycemic effect is exerted, this research explored the influence of the extracts of the crude stem-bark of C. cassia, with various polarities, on particular aspects of glucose consumption as well as glucose-stimulated insulin synthesis and secretion by RIN-m5F cells. All C. cassia extracts did not enhance glucose consumption when compared to the untreated cells (p˂0.001). However, lower concentrations of the extracts significantly increased mRNA levels of Glucose Transporter 2 (GLUT2) and glucokinase genes. Furthermore, all C. cassia extracts significantly stimulated/increased total insulin synthesis (intracellular and secreted) when compared with the control cells, though the effect was not dose-dependent except for water extracts at higher concentration (p<0.001). The results of this investigation revealed that generally, the extracts upregulated the expression of Musculoaponeurotic fibrosarcoma homolog A (MafA), the transcription factor involved in insulin gene expression, while that of the Pancreatic Duodenal Homeobox-1 (PDX-1) gene was mostly suppressed. In concluding, the results of this study proposes that C. cassia stem-bark extracts may exert hypoglycemic effects through stimulating insulin synthesis and secretion in the RIN-m5F pancreatic beta cells.
AB - Cinnamomum cassia (C. cassia) crude stem-bark extracts possess some hypoglycemic properties through inhibiting the uptake of glucose by the intestines and/or enhancing insulin sensitivity. To further investigate the mechanism through which the hypoglycemic effect is exerted, this research explored the influence of the extracts of the crude stem-bark of C. cassia, with various polarities, on particular aspects of glucose consumption as well as glucose-stimulated insulin synthesis and secretion by RIN-m5F cells. All C. cassia extracts did not enhance glucose consumption when compared to the untreated cells (p˂0.001). However, lower concentrations of the extracts significantly increased mRNA levels of Glucose Transporter 2 (GLUT2) and glucokinase genes. Furthermore, all C. cassia extracts significantly stimulated/increased total insulin synthesis (intracellular and secreted) when compared with the control cells, though the effect was not dose-dependent except for water extracts at higher concentration (p<0.001). The results of this investigation revealed that generally, the extracts upregulated the expression of Musculoaponeurotic fibrosarcoma homolog A (MafA), the transcription factor involved in insulin gene expression, while that of the Pancreatic Duodenal Homeobox-1 (PDX-1) gene was mostly suppressed. In concluding, the results of this study proposes that C. cassia stem-bark extracts may exert hypoglycemic effects through stimulating insulin synthesis and secretion in the RIN-m5F pancreatic beta cells.
KW - Cinnamomum cassia
KW - Diabetes
KW - Glucose Uptake
KW - MafA and PDX-1
KW - Pancreatic Beta Cells
KW - Synthesis and Secretion of Insulin
UR - http://www.scopus.com/inward/record.url?scp=85128629612&partnerID=8YFLogxK
U2 - 10.3844/ojbsci.2022.178.191
DO - 10.3844/ojbsci.2022.178.191
M3 - Article
AN - SCOPUS:85128629612
SN - 1608-4217
VL - 22
SP - 178
EP - 191
JO - OnLine Journal of Biological Sciences
JF - OnLine Journal of Biological Sciences
IS - 2
ER -