TY - JOUR
T1 - Effect of streptozocin-induced diabetes on the histomorphometry of the liver and kidneys of male sprague dawley rats
AU - Mbelengwa, Pfarelo A.
AU - Mpholwane, Matome L.
AU - Xhakaza, Nkosi
N1 - Publisher Copyright:
© 2024 Author(s). This is an open access, peer-reviewed article published by Firenze University Press (https://www.fupress.com) and distributed, except where otherwise noted, under the terms of the CC BY 4.0 License for content and CC0 1.0 Universal for metadata.
PY - 2025
Y1 - 2025
N2 - The liver and kidneys are among primary organs affected by Diabetes mellitus (DM), whereas chronic antidiabetic medication has side effects on these organs. Due to the said side effects, there is an increase in research for new natural based antidiabetic medication making use of streptozocin (STZ) induced diabetic rodent model. However, dosage and duration of STZ in diabetes induction vary with potential inconsistencies in interpretation of the results by different authors. We investigated the effects of a single dose, (50mg/kg) of STZ on the histomorphometry of liver and kidneys of male Sprague Dawley rats after 21 days of diabetes induction. Hepatocyte (HA), nuclear and cytoplasmic (CA) areas were measured in zones 1 and 3 of the liver tissues from 16 [8 Normal C, 8 STZ diabetic DM] male Sprague Dawley rats. Corpuscular, renal, glomerular tuft, tubular, epithelial, luminal areas and connective tissue were also measured in kidney tubules from the same animals using a hand tool of ImageJ software. Means were compared using a student’s t-test in SPSS software. HA in zone1 of DM was significantly higher than that of the C (p=0.009), while HA in zone 3 of DM were significantly lower than in C (p=0.032). The CA in zone1 of DM was significantly higher than that of C (p=0.006). A significant change was only fibrosis of the glomerular tuft in kidneys. 50mg/kg STZ induced diabetes caused some changes in the liver and kidney tissues but not a full pathologic profile as seen in studies with a longer duration.
AB - The liver and kidneys are among primary organs affected by Diabetes mellitus (DM), whereas chronic antidiabetic medication has side effects on these organs. Due to the said side effects, there is an increase in research for new natural based antidiabetic medication making use of streptozocin (STZ) induced diabetic rodent model. However, dosage and duration of STZ in diabetes induction vary with potential inconsistencies in interpretation of the results by different authors. We investigated the effects of a single dose, (50mg/kg) of STZ on the histomorphometry of liver and kidneys of male Sprague Dawley rats after 21 days of diabetes induction. Hepatocyte (HA), nuclear and cytoplasmic (CA) areas were measured in zones 1 and 3 of the liver tissues from 16 [8 Normal C, 8 STZ diabetic DM] male Sprague Dawley rats. Corpuscular, renal, glomerular tuft, tubular, epithelial, luminal areas and connective tissue were also measured in kidney tubules from the same animals using a hand tool of ImageJ software. Means were compared using a student’s t-test in SPSS software. HA in zone1 of DM was significantly higher than that of the C (p=0.009), while HA in zone 3 of DM were significantly lower than in C (p=0.032). The CA in zone1 of DM was significantly higher than that of C (p=0.006). A significant change was only fibrosis of the glomerular tuft in kidneys. 50mg/kg STZ induced diabetes caused some changes in the liver and kidney tissues but not a full pathologic profile as seen in studies with a longer duration.
KW - diabetes mellitus
KW - diabetic nephropathy
KW - kidneys
KW - liver
KW - steatohepatitis
UR - https://www.scopus.com/pages/publications/105010599002
U2 - 10.36253/ijae-15806
DO - 10.36253/ijae-15806
M3 - Article
AN - SCOPUS:105010599002
SN - 1122-6714
VL - 129
SP - 29
EP - 39
JO - Italian Journal of Anatomy and Embryology
JF - Italian Journal of Anatomy and Embryology
IS - 1
ER -
