Electropherotypes and subgroups of group A rotaviruses circulating among diarrhoeic children in Kano, Nigeria

A. A. Dzikwi*, J. U. Umoh, J. K.P. Kwaga, A. A. Ahmad, M. deBeer, A. D. Steele

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: It is estimated that about 600 000 children die annually as a result of severe dehydrating diarrhea caused by rotaviruses. The virus is a double stranded RNA (dsRNA) virus with 11 segments. Group A rotaviruses show a characteristic 4-2-3-2 pattern following electrophoresis. The VP6 subgroups, I and II exist. This work was carried out to study the prevalence of rotavirus infection among children 0-5 years with diarrhea in Kano, and to determine the circulating subgroups and electropherotypes and of the rotavirus isolates. Methods: Two hundred and eighteen stool specimens from children 0-60 months (198 diarrheic and 20 non-diarrheic) were collected from different hospitals and health care centers in Kano and subjected to group A rotavirus enzyme linked immunosorbent assay (ELISA) to determine presence of group A rotavirus, subgroup ELISA to determine the VP6 subgroups and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes present. Results: The long electropherotypes (47.05%) of four variations dominated over the short electropherotype (17.64%). About 11.76% of the isolates were of mixed infection. Dominance of subgroup II (45%) over subgroup I (25%), and the presence of both subgroups I and II (10%) and neither subgroup I nor II (15%) was observed in this study. Conclusion: Information on the genomic diversity of the RNA electropherotypes in this region, Kano, is reported in this study.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalAnnals of African Medicine
Volume7
Issue number4
DOIs
Publication statusPublished - 2008

Keywords

  • Eletropherotypes
  • Group A
  • Nigeria
  • Rotavirus
  • Subgroups

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