Entamoeba histolytica: Genetic diversity of African strains based on the polymorphism of the serine-rich protein gene

A. Samie; C. L. Obi; P. O. Bessong; E. Houpt; S. Stroup; M. Njayou; C. Sabeta; T. Mduluza; R. L. Guerrant

doi:10.1016/j.exppara.2007.09.008

Entamoeba histolytica: Genetic diversity of African strains based on the polymorphism of the serine-rich protein gene

A. Samie^*, C. L. Obi, P. O. Bessong, E. Houpt, S. Stroup, M. Njayou, C. Sabeta, T. Mduluza, R. L. Guerrant

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The polymorphism of the serine-rich Entamoeba histolytica protein (SREHP) among isolates obtained from different geographic regions was analyzed by a nested PCR followed by restriction analysis. Thirteen different profiles were generated from 23 E. histolytica isolates from Cameroon, Zimbabwe and South Africa while 20 others were generated from 38 E. histolytica PCR positive stool samples from South Africa. One of the profiles was common to isolates from Cameroon, Zimbabwe and South Africa and constituted the most prevalent (26.1%) of all the profiles. However, profiles unique to each country were also observed amongst the samples. A non-significant difference was observed between isolates from diarrheic and non-diarrheic samples. Of interest, of the five HIV positive stool samples three had the same profile indicating the possibility that some E. histolytica strains might be more common/pathogenic in immuno-compromised individuals. The results obtained showed that African isolates of E. histolytica may possess extremely complex genetic structures independent of geographic location. This study indicates that certain profiles might be responsible for the presentation of intestinal amoebic symptoms. However, more extended studies need to be performed in order to confirm these observations.

Original language	English
Pages (from-to)	354-361
Number of pages	8
Journal	Experimental Parasitology
Volume	118
Issue number	3
DOIs	https://doi.org/10.1016/j.exppara.2007.09.008
Publication status	Published - Mar 2008
Externally published	Yes

Keywords

Africa
Diarrhea
Entamoeba histolytica
Genetic diversity
Intestinal inflammation
Occult blood

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exppara.2007.09.008

Cite this

@article{8655fde33df6430b912d7e7b88b6f46c,

title = "Entamoeba histolytica: Genetic diversity of African strains based on the polymorphism of the serine-rich protein gene",

abstract = "The polymorphism of the serine-rich Entamoeba histolytica protein (SREHP) among isolates obtained from different geographic regions was analyzed by a nested PCR followed by restriction analysis. Thirteen different profiles were generated from 23 E. histolytica isolates from Cameroon, Zimbabwe and South Africa while 20 others were generated from 38 E. histolytica PCR positive stool samples from South Africa. One of the profiles was common to isolates from Cameroon, Zimbabwe and South Africa and constituted the most prevalent (26.1%) of all the profiles. However, profiles unique to each country were also observed amongst the samples. A non-significant difference was observed between isolates from diarrheic and non-diarrheic samples. Of interest, of the five HIV positive stool samples three had the same profile indicating the possibility that some E. histolytica strains might be more common/pathogenic in immuno-compromised individuals. The results obtained showed that African isolates of E. histolytica may possess extremely complex genetic structures independent of geographic location. This study indicates that certain profiles might be responsible for the presentation of intestinal amoebic symptoms. However, more extended studies need to be performed in order to confirm these observations.",

keywords = "Africa, Diarrhea, Entamoeba histolytica, Genetic diversity, Intestinal inflammation, Occult blood",

author = "A. Samie and Obi, {C. L.} and Bessong, {P. O.} and E. Houpt and S. Stroup and M. Njayou and C. Sabeta and T. Mduluza and Guerrant, {R. L.}",

note = "Funding Information: Support for this study was provided by the Ellison Medical Foundation{\textquoteright}s grant to the Centre for Global Health, University of Virginia, Charlottesville, VA, USA. Mr. A.S. was fellow of the The United Nations Educational, Scientific and Cultural Organization (UNESCO) Biotechnology Action Council (BAC) programme for part of this study. The authors are also grateful to the officials of the primary schools and the hospitals for their collaboration in the sample collection.",

year = "2008",

month = mar,

doi = "10.1016/j.exppara.2007.09.008",

language = "English",

volume = "118",

pages = "354--361",

journal = "Experimental Parasitology",

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TY - JOUR

T1 - Entamoeba histolytica

T2 - Genetic diversity of African strains based on the polymorphism of the serine-rich protein gene

AU - Samie, A.

AU - Obi, C. L.

AU - Bessong, P. O.

AU - Houpt, E.

AU - Stroup, S.

AU - Njayou, M.

AU - Sabeta, C.

AU - Mduluza, T.

AU - Guerrant, R. L.

N1 - Funding Information: Support for this study was provided by the Ellison Medical Foundation’s grant to the Centre for Global Health, University of Virginia, Charlottesville, VA, USA. Mr. A.S. was fellow of the The United Nations Educational, Scientific and Cultural Organization (UNESCO) Biotechnology Action Council (BAC) programme for part of this study. The authors are also grateful to the officials of the primary schools and the hospitals for their collaboration in the sample collection.

PY - 2008/3

Y1 - 2008/3

N2 - The polymorphism of the serine-rich Entamoeba histolytica protein (SREHP) among isolates obtained from different geographic regions was analyzed by a nested PCR followed by restriction analysis. Thirteen different profiles were generated from 23 E. histolytica isolates from Cameroon, Zimbabwe and South Africa while 20 others were generated from 38 E. histolytica PCR positive stool samples from South Africa. One of the profiles was common to isolates from Cameroon, Zimbabwe and South Africa and constituted the most prevalent (26.1%) of all the profiles. However, profiles unique to each country were also observed amongst the samples. A non-significant difference was observed between isolates from diarrheic and non-diarrheic samples. Of interest, of the five HIV positive stool samples three had the same profile indicating the possibility that some E. histolytica strains might be more common/pathogenic in immuno-compromised individuals. The results obtained showed that African isolates of E. histolytica may possess extremely complex genetic structures independent of geographic location. This study indicates that certain profiles might be responsible for the presentation of intestinal amoebic symptoms. However, more extended studies need to be performed in order to confirm these observations.

AB - The polymorphism of the serine-rich Entamoeba histolytica protein (SREHP) among isolates obtained from different geographic regions was analyzed by a nested PCR followed by restriction analysis. Thirteen different profiles were generated from 23 E. histolytica isolates from Cameroon, Zimbabwe and South Africa while 20 others were generated from 38 E. histolytica PCR positive stool samples from South Africa. One of the profiles was common to isolates from Cameroon, Zimbabwe and South Africa and constituted the most prevalent (26.1%) of all the profiles. However, profiles unique to each country were also observed amongst the samples. A non-significant difference was observed between isolates from diarrheic and non-diarrheic samples. Of interest, of the five HIV positive stool samples three had the same profile indicating the possibility that some E. histolytica strains might be more common/pathogenic in immuno-compromised individuals. The results obtained showed that African isolates of E. histolytica may possess extremely complex genetic structures independent of geographic location. This study indicates that certain profiles might be responsible for the presentation of intestinal amoebic symptoms. However, more extended studies need to be performed in order to confirm these observations.

KW - Africa

KW - Diarrhea

KW - Entamoeba histolytica

KW - Genetic diversity

KW - Intestinal inflammation

KW - Occult blood

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DO - 10.1016/j.exppara.2007.09.008

M3 - Article

C2 - 18028911

AN - SCOPUS:38749109266

SN - 0014-4894

VL - 118

SP - 354

EP - 361

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 3

ER -

Entamoeba histolytica: Genetic diversity of African strains based on the polymorphism of the serine-rich protein gene

Abstract

Keywords

UN SDGs

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