Enteroaggregative Escherichia coli in Venda, South Africa: Distribution of virulence-related genes by multiplex polymerase chain reaction in stool samples of human immunodeficiency virus (HIV)-positive and HIV-negative individuals and primary school children

Amidou Samie*, Chikwelu Larry Obi, Rebecca Dillingham, Relana C. Pinkerton, Richard L. Guerrant

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We used a multiplex polymerase chain reaction (PCR) and a quantitative real-time PCR to determine the distribution of three enteroaggregative Escherichia coli (EAEC) virulence-related genes in stool samples from hospital patients and school children in the Venda region of South Africa. At least one gene was found in 52 (16.5%) samples, 50 (19.6%) from hospitals and 2 (3%) from schools. The AA probe was found in 36 (69%), the aggR gene was found in 41 (79%), and the aap gene was found in 49 (94%) of all positive samples. EAEC was significantly associated with diarrhea and intestinal inflammation and was significantly higher (χ2 = 5.360, P = 0.021) in human immunodeficiency virus (HIV)-positive persons (29.5%) than in HIV-negative persons (13.7%). The presence of EAEC genes was significantly associated with occult blood (χ2 = 30.543, P < 0.0001) in the stool samples. This study suggests that the clinical presentation of EAEC infection may be directly related to the bacterial load as well as to the genetic characteristics of the strains involved.

Original languageEnglish
Pages (from-to)142-150
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume77
Issue number1
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

Fingerprint

Dive into the research topics of 'Enteroaggregative Escherichia coli in Venda, South Africa: Distribution of virulence-related genes by multiplex polymerase chain reaction in stool samples of human immunodeficiency virus (HIV)-positive and HIV-negative individuals and primary school children'. Together they form a unique fingerprint.

Cite this