Evaluation of selected South African medicinal plants for inhibitory properties against human immunodeficiency virus type 1 reverse transcriptase and integrase

Pascal Obong Bessong*, Chikwelu Larry Obi, Marie Line Andréola, Luis B. Rojas, Laurent Pouységu, Eunice Igumbor, J. J.Marion Meyer, Stéphane Quideau, Simon Litvak

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

Seventeen aqueous and methanol extracts from nine South African medicinal plants, ethnobotanically selected, were screened for inhibitory properties against HIV-1 reverse transcriptase (RT). Isolated compounds were additionally evaluated on HIV-1 integrase (IN). The strongest inhibition against the RNA-dependent-DNA polymerase (RDDP) activity of RT was observed with the methanol extract of the stem-bark of Peltophorum africanum Sond. (Fabaceae) (IC50 3.5 μg/ml), while the methanol extract of the roots of Combretum molle R.Br. ex G. Don (Combretaceae) was the most inhibitory on the ribonuclease H (RNase H) activity (IC50 9.7 μg/ml). The known compounds bergenin and catechin, and a red coloured gallotannin composed of meta-depside chains of gallic and protocatechuic acids esterified to a 1-O-isobutyroly-β-d-glucopyranose core, were isolated from the methanol extract of the roots and stem-bark of Peltophorum africanum. The gallotannin inhibited the RDDP and RNase H functions of RT with IC50 values of 6.0 and 5.0 μM, respectively, and abolished the 3′-end processing activity of IN at 100 μM. Catechin showed no effect on RT but had a moderate activity on HIV-1 IN. Bergenin was inactive on both enzymes. The aqueous and methanol extracts were non-toxic in a HeLaP4 cell line at a concentration of 400 μg/ml.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalJournal of Ethnopharmacology
Volume99
Issue number1
DOIs
Publication statusPublished - 13 May 2005
Externally publishedYes

Keywords

  • Gallotannins
  • HIV-1
  • Integrase
  • Plant phenols
  • Reverse transcriptase
  • South African medicinal plants

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