TY - JOUR
T1 - Fermentable carbohydrate alters hypothalamic neuronal activity and protects against the obesogenic environment
AU - Anastasovska, Jelena
AU - Arora, Tulika
AU - Sanchez Canon, Gina J.
AU - Parkinson, James R.C.
AU - Touhy, Kieran
AU - R. Gibson, Glen
AU - Nadkarni, Nachiket A.
AU - So, Po Wah
AU - Goldstone, Anthony P.
AU - Thomas, E. Louise
AU - K. Hankir, Mohammed
AU - Van Loo, Jan
AU - Modi, Neena
AU - Bell, Jimmy D.
AU - Frost, Gary
PY - 2012/5
Y1 - 2012/5
N2 - Obesity has become a major global health problem. Recently, attention has focused on the benefits of fermentable carbohydrates on modulating metabolism. Here, we take a system approach to investigate the physiological effects of supplementation with oligofructose-enriched inulin (In). We hypothesize that supplementation with this fermentable carbohydrate will not only lead to changes in body weight and composition, but also to modulation in neuronal activation in the hypothalamus. Male C57BL/6 mice were maintained on a normal chow diet (control) or a high fat (HF) diet supplemented with either oligofructose- enriched In or corn starch (Cs) for 9 weeks. Compared to HFCs diet, In supplementation led to significant reduction in average daily weight gain (mean±s.e.m.: 0.19±0.01 g vs. 0.26±0.02 g, P<0.01), total body adiposity (24.9±1.2% vs. 30.7±1.4%, P <0.01), and lowered liver fat content (11.7±1.7% vs. 23.8±3.4%, P <0.01). Significant changes were also observed in fecal bacterial distribution, with increases in both Bifidobacteria and Lactobacillius and a significant increase in short chain fatty acids (SCFA). Using manganese-enhanced MRI (MEMRI), we observed a significant increase in neuronal activation within the arcuate nucleus (ARC) of animals that received In supplementation compared to those fed HFCs diet. In conclusion, we have demonstrated for the first time, in the same animal, a wide range of beneficial metabolic effects following supplementation of a HF diet with oligofructose-enriched In, as well as significant changes in hypothalamic neuronal activity.
AB - Obesity has become a major global health problem. Recently, attention has focused on the benefits of fermentable carbohydrates on modulating metabolism. Here, we take a system approach to investigate the physiological effects of supplementation with oligofructose-enriched inulin (In). We hypothesize that supplementation with this fermentable carbohydrate will not only lead to changes in body weight and composition, but also to modulation in neuronal activation in the hypothalamus. Male C57BL/6 mice were maintained on a normal chow diet (control) or a high fat (HF) diet supplemented with either oligofructose- enriched In or corn starch (Cs) for 9 weeks. Compared to HFCs diet, In supplementation led to significant reduction in average daily weight gain (mean±s.e.m.: 0.19±0.01 g vs. 0.26±0.02 g, P<0.01), total body adiposity (24.9±1.2% vs. 30.7±1.4%, P <0.01), and lowered liver fat content (11.7±1.7% vs. 23.8±3.4%, P <0.01). Significant changes were also observed in fecal bacterial distribution, with increases in both Bifidobacteria and Lactobacillius and a significant increase in short chain fatty acids (SCFA). Using manganese-enhanced MRI (MEMRI), we observed a significant increase in neuronal activation within the arcuate nucleus (ARC) of animals that received In supplementation compared to those fed HFCs diet. In conclusion, we have demonstrated for the first time, in the same animal, a wide range of beneficial metabolic effects following supplementation of a HF diet with oligofructose-enriched In, as well as significant changes in hypothalamic neuronal activity.
UR - http://www.scopus.com/inward/record.url?scp=84860297167&partnerID=8YFLogxK
U2 - 10.1038/oby.2012.6
DO - 10.1038/oby.2012.6
M3 - Article
C2 - 22322344
AN - SCOPUS:84860297167
SN - 1930-7381
VL - 20
SP - 1016
EP - 1023
JO - Obesity
JF - Obesity
IS - 5
ER -
