Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

Amy Strydom, Celeste Donato, Ina Peenze, A. Christiaan Potgieter, Mapaseka Seheri, Hester G. O'Neill*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animals.

Original languageEnglish
Article number104463
JournalInfection, Genetics and Evolution
Volume85
DOIs
Publication statusPublished - Nov 2020

Keywords

  • African buffalo
  • Artiodactyl backbone
  • G29
  • P[14]
  • Rotavirus type A

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