Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

Amy Strydom; Celeste Donato; Ina Peenze; A. Christiaan Potgieter; Mapaseka Seheri; Hester G. O'Neill

doi:10.1016/j.meegid.2020.104463

Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

Amy Strydom, Celeste Donato, Ina Peenze, A. Christiaan Potgieter, Mapaseka Seheri, Hester G. O'Neill^*

^*Corresponding author for this work

Virological Pathology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animals.

Original language	English
Article number	104463
Journal	Infection, Genetics and Evolution
Volume	85
DOIs	https://doi.org/10.1016/j.meegid.2020.104463
Publication status	Published - Nov 2020

Keywords

African buffalo
Artiodactyl backbone
G29
P[14]
Rotavirus type A

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.meegid.2020.104463

Cite this

@article{0f702abf2bdd4c3b8f016738f0fbcfd3,

title = "Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo",

abstract = "We report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animals.",

keywords = "African buffalo, Artiodactyl backbone, G29, P[14], Rotavirus type A",

author = "Amy Strydom and Celeste Donato and Ina Peenze and Potgieter, {A. Christiaan} and Mapaseka Seheri and O'Neill, {Hester G.}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = nov,

doi = "10.1016/j.meegid.2020.104463",

language = "English",

volume = "85",

journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

AU - Strydom, Amy

AU - Donato, Celeste

AU - Peenze, Ina

AU - Potgieter, A. Christiaan

AU - Seheri, Mapaseka

AU - O'Neill, Hester G.

PY - 2020/11

Y1 - 2020/11

N2 - We report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animals.

AB - We report the first description of rotavirus A strains in African buffalo (Syncerus caffer). Following RNA extraction from stool samples, cDNA was prepared, followed either by sequence-independent amplification and 454 pyrosequencing or direct sequencing on an Illumina MiSeq platform. RVA/Buffalo-wt/ZAF/4426/2002/G29P[14] exhibited a novel G29P[14] combination and an artiodactyl backbone: I2-R2-C2-M2-A11-N2-T6-E2-H3. RVA/Buffalo-wt/ZAF/1442/2007/G10P[11] also exhibited an artiodactyl backbone: I2-R2-C2-M2-A13-N2-T6-E2-H3. Characterisation of these genome constellations indicate that the two buffalo strains are moderately diverse from each other and related to South African bovine RVA strains. The detection of RVA in buffalo contribute to our understanding of the host range of rotavirus in animals.

KW - African buffalo

KW - Artiodactyl backbone

KW - G29

KW - P[14]

KW - Rotavirus type A

UR - http://www.scopus.com/inward/record.url?scp=85088216008&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2020.104463

DO - 10.1016/j.meegid.2020.104463

M3 - Article

C2 - 32693063

AN - SCOPUS:85088216008

SN - 1567-1348

VL - 85

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

M1 - 104463

ER -

Genetic characterisation of novel G29P[14] and G10P[11] rotavirus strains from African buffalo

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this