TY - JOUR
T1 - Genetic characterization of HIV before widespread testing of HIV vaccine candidates at a clinical trial site in Pretoria, South Africa
AU - Musyoki, Andrew Munyalo
AU - Mothapo, Khutso
AU - Rakgole, Jonny Nare
AU - Lukhwareni, Azwidowi
AU - Bessong, Pascal
AU - Selabe, Selokela Gloria
AU - Bredell, Helba
AU - Williamson, Carolyn
AU - Mphahlele, M. Jeffrey
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2012/9/1
Y1 - 2012/9/1
N2 - We studied 123 samples from adult chronic HIV patients initiating HAART from various centers around a newly established clinical trial site in Pretoria. Each sample was sequenced in at least one structural gene (pol, gag, and env) or functional gene (vif, vpr, and vpu). A subset of 25 samples was subjected to near full-genome analysis. All samples were HIV-1 subtype C. Highly conserved regions within the gene sequences were observed. Overall, the gag and vif sequences showed closer similarity followed by the env, vpr, pol, and vpu. The env gene was the most difficult to sequence, resulting in only 31 sequences from 40 samples; of these, 25 were predicted to be R5 coreceptor tropic, while 6 were X4 tropic. The study asserted the predominance of HIV-1 subtype C within the catchment population.
AB - We studied 123 samples from adult chronic HIV patients initiating HAART from various centers around a newly established clinical trial site in Pretoria. Each sample was sequenced in at least one structural gene (pol, gag, and env) or functional gene (vif, vpr, and vpu). A subset of 25 samples was subjected to near full-genome analysis. All samples were HIV-1 subtype C. Highly conserved regions within the gene sequences were observed. Overall, the gag and vif sequences showed closer similarity followed by the env, vpr, pol, and vpu. The env gene was the most difficult to sequence, resulting in only 31 sequences from 40 samples; of these, 25 were predicted to be R5 coreceptor tropic, while 6 were X4 tropic. The study asserted the predominance of HIV-1 subtype C within the catchment population.
UR - http://www.scopus.com/inward/record.url?scp=84865462657&partnerID=8YFLogxK
U2 - 10.1089/aid.2011.0285
DO - 10.1089/aid.2011.0285
M3 - Article
C2 - 22106990
SN - 0889-2229
VL - 28
SP - 931
EP - 938
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 9
ER -