Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: Relationship to animal rotaviruses

M. D. Esona; K. Banyai; K. Foytich; M. Freeman; S. Mijatovic-Rustempasic; J. Hull; T. Kerin; A. D. Steele; G. E. Armah; A. Geyer; N. Page; V. A. Agbaya; J. C. Forbi; M. Aminu; R. Gautam; L. M. Seheri; J. Nyangao; R. Glass; M. D. Bowen; J. R. Gentsch

doi:10.1016/j.meegid.2010.09.010

Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: Relationship to animal rotaviruses

M. D. Esona^*, K. Banyai, K. Foytich, M. Freeman, S. Mijatovic-Rustempasic, J. Hull, T. Kerin, A. D. Steele, G. E. Armah, A. Geyer, N. Page, V. A. Agbaya, J. C. Forbi, M. Aminu, R. Gautam, L. M. Seheri, J. Nyangao, R. Glass, M. D. Bowen, J. R. Gentsch

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Global rotavirus surveillance has led to the detection of many unusual human rotavirus (HRV) genotypes. The aim of this study was to elucidate the genetic and evolutionary relationships of short fragments of all 11 gene segments of G10 HRV strains identified in West Africa through the African Rotavirus Network (ARN) system. During 1998-2004 surveillance within the ARN, we identified 5 G10 P[8] HRV strains. Fragments of all 11 gene segments of these G10 strains were sequenced. Phylogenetic and sequence analyses of each gene segment revealed high nucleotide similarities amongst the ARN strains (97-100%) except in the case of the VP1(85-96%) and NSP2 genes (87.8-99.7%) where some strains were divergent. All genes of the ARN strains were classified as Wa-like (genotype 1) with the exception of their VP7 gene of all strains (genotype G10) and the VP6 gene of a single strain, 6755/2002/ARN (DS-1 like, genotype 2). While classified as Wa-like, the NSP2 genes of four of the ARN strains occupied a distinct sub-lineage related to simian strain Tuch, while the NSP2 of strain 6755/2002/ARN and NSP5 genes of all strains were closely related to the cognate genes of both human and animal strains belonging to the Wa-like genogroup. Although these findings help to elucidate the evolution of ARN G10 strains, additional sequence studies of cognate animal rotavirus genes are needed to determine irrefutably the specific origin of those genes relative to both human and animal rotavirus strains.

Original language	English
Pages (from-to)	237-241
Number of pages	5
Journal	Infection, Genetics and Evolution
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/j.meegid.2010.09.010
Publication status	Published - Jan 2011
Externally published	Yes

Keywords

Gastroenteritis
Genome configuration
Reassortment
Zoonotic origin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.meegid.2010.09.010

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Esona, M. D., Banyai, K., Foytich, K., Freeman, M., Mijatovic-Rustempasic, S., Hull, J., Kerin, T., Steele, A. D., Armah, G. E., Geyer, A., Page, N., Agbaya, V. A., Forbi, J. C., Aminu, M., Gautam, R., Seheri, L. M., Nyangao, J., Glass, R., Bowen, M. D., & Gentsch, J. R. (2011). Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: Relationship to animal rotaviruses. Infection, Genetics and Evolution, 11(1), 237-241. https://doi.org/10.1016/j.meegid.2010.09.010

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abstract = "Global rotavirus surveillance has led to the detection of many unusual human rotavirus (HRV) genotypes. The aim of this study was to elucidate the genetic and evolutionary relationships of short fragments of all 11 gene segments of G10 HRV strains identified in West Africa through the African Rotavirus Network (ARN) system. During 1998-2004 surveillance within the ARN, we identified 5 G10 P[8] HRV strains. Fragments of all 11 gene segments of these G10 strains were sequenced. Phylogenetic and sequence analyses of each gene segment revealed high nucleotide similarities amongst the ARN strains (97-100%) except in the case of the VP1(85-96%) and NSP2 genes (87.8-99.7%) where some strains were divergent. All genes of the ARN strains were classified as Wa-like (genotype 1) with the exception of their VP7 gene of all strains (genotype G10) and the VP6 gene of a single strain, 6755/2002/ARN (DS-1 like, genotype 2). While classified as Wa-like, the NSP2 genes of four of the ARN strains occupied a distinct sub-lineage related to simian strain Tuch, while the NSP2 of strain 6755/2002/ARN and NSP5 genes of all strains were closely related to the cognate genes of both human and animal strains belonging to the Wa-like genogroup. Although these findings help to elucidate the evolution of ARN G10 strains, additional sequence studies of cognate animal rotavirus genes are needed to determine irrefutably the specific origin of those genes relative to both human and animal rotavirus strains.",

keywords = "Gastroenteritis, Genome configuration, Reassortment, Zoonotic origin",

author = "Esona, {M. D.} and K. Banyai and K. Foytich and M. Freeman and S. Mijatovic-Rustempasic and J. Hull and T. Kerin and Steele, {A. D.} and Armah, {G. E.} and A. Geyer and N. Page and Agbaya, {V. A.} and Forbi, {J. C.} and M. Aminu and R. Gautam and Seheri, {L. M.} and J. Nyangao and R. Glass and Bowen, {M. D.} and Gentsch, {J. R.}",

year = "2011",

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doi = "10.1016/j.meegid.2010.09.010",

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Esona, MD, Banyai, K, Foytich, K, Freeman, M, Mijatovic-Rustempasic, S, Hull, J, Kerin, T, Steele, AD, Armah, GE, Geyer, A, Page, N, Agbaya, VA, Forbi, JC, Aminu, M, Gautam, R, Seheri, LM, Nyangao, J, Glass, R, Bowen, MD & Gentsch, JR 2011, 'Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: Relationship to animal rotaviruses', Infection, Genetics and Evolution, vol. 11, no. 1, pp. 237-241. https://doi.org/10.1016/j.meegid.2010.09.010

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T1 - Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network

T2 - Relationship to animal rotaviruses

AU - Esona, M. D.

AU - Banyai, K.

AU - Foytich, K.

AU - Freeman, M.

AU - Mijatovic-Rustempasic, S.

AU - Hull, J.

AU - Kerin, T.

AU - Steele, A. D.

AU - Armah, G. E.

AU - Geyer, A.

AU - Page, N.

AU - Agbaya, V. A.

AU - Forbi, J. C.

AU - Aminu, M.

AU - Gautam, R.

AU - Seheri, L. M.

AU - Nyangao, J.

AU - Glass, R.

AU - Bowen, M. D.

AU - Gentsch, J. R.

PY - 2011/1

Y1 - 2011/1

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AB - Global rotavirus surveillance has led to the detection of many unusual human rotavirus (HRV) genotypes. The aim of this study was to elucidate the genetic and evolutionary relationships of short fragments of all 11 gene segments of G10 HRV strains identified in West Africa through the African Rotavirus Network (ARN) system. During 1998-2004 surveillance within the ARN, we identified 5 G10 P[8] HRV strains. Fragments of all 11 gene segments of these G10 strains were sequenced. Phylogenetic and sequence analyses of each gene segment revealed high nucleotide similarities amongst the ARN strains (97-100%) except in the case of the VP1(85-96%) and NSP2 genes (87.8-99.7%) where some strains were divergent. All genes of the ARN strains were classified as Wa-like (genotype 1) with the exception of their VP7 gene of all strains (genotype G10) and the VP6 gene of a single strain, 6755/2002/ARN (DS-1 like, genotype 2). While classified as Wa-like, the NSP2 genes of four of the ARN strains occupied a distinct sub-lineage related to simian strain Tuch, while the NSP2 of strain 6755/2002/ARN and NSP5 genes of all strains were closely related to the cognate genes of both human and animal strains belonging to the Wa-like genogroup. Although these findings help to elucidate the evolution of ARN G10 strains, additional sequence studies of cognate animal rotavirus genes are needed to determine irrefutably the specific origin of those genes relative to both human and animal rotavirus strains.

KW - Gastroenteritis

KW - Genome configuration

KW - Reassortment

KW - Zoonotic origin

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IS - 1

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Genomic characterization of human rotavirus G10 strains from the African Rotavirus Network: Relationship to animal rotaviruses

Abstract

Keywords

UN SDGs

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