Increased bioconversion of pretreated wastepaper to sugars by successive treatment with cellulase from Penicillium funiculosum and Trichoderma reesei

Used paper materials were treated with cellulase from Penicillium funiculosum and Trichoderma reesei to bioconvert their cellulose component to reducing sugars. Saccharification was increased by milling this wastepaper into a fine powder prior to cellulase catalyzed hydrolysis and successive treatment with fresh cellulase also exceeded prolonged degradation. Maximum bioconversion was observed when T. reesei cellulase was used as the initial enzyme system during successive hydrolyses of pretreated foolscap paper and filter paper.