La exposición al alcohol intrauterino altera la morfología trabecular en la epífisis del húmero de la rata sprague dawley hasta 3 semanas después del nacimiento

Translated title of the contribution: Intrauterine alcohol exposure disturbs trabecular morphology in the sprague dawley rat humerus epiphysis up to 3-weeks postnatally

Diana Pillay*, Robert Ndou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

SUMMARY: Gestational alcohol exposure inhibits neurological as well as bone growth and development both in fetal and postnatal life. Stunted stature, osteoporosis and fractures in adult life are some of the adverse effects. While the impact of intrauterine alcohol on the brain has been extensively investigated, studies on the effects on bone are relatively few. Therefore, our study aimed to examine the impact of prenatal alcohol exposure on bone microarchitecture in 3-week-old rats using Micro-focus X-Ray Computed Tomography (Micro CT). Time mated pregnant Sprague Dawley dams (13) were randomly placed into 3 groups: ethanol (n=5), saline control (n=5) and untreated control (n=3). The former 2 groups received treatment with 0.015ml/g of 25.2 % ethanol and 0.9 % saline, respectively, for the first 19 days of gestation. The untreated group received no treatment. The pups remained with their dams until termination at 21 days of age. From each dam, 2 pups were collected resulting in: ethanol (n=10), saline controls (n= 10) and untreated controls (n = 6). The humeri of the pups were dissected and scanned using a 3D-µCT scanner (Nikon XTH 225L) at 15µm resolution. Trabecular and cortical parameters were analysed using Volume Graphics Studio® software following reconstruction. Results showed a decrease in trabecular size, spaces, thickness, and volume. There was a decrease in cortical bone area in the ethanol group compared to the controls. These findings may suggest that osteoporosis and fractures seen as gestational alcohol effects may be due to compromised trabecular structure.

Translated title of the contributionIntrauterine alcohol exposure disturbs trabecular morphology in the sprague dawley rat humerus epiphysis up to 3-weeks postnatally
Original languageSpanish
Pages (from-to)1436-1442
Number of pages7
JournalInternational Journal of Morphology
Volume39
Issue number5
DOIs
Publication statusPublished - Oct 2021

Keywords

  • Humerus
  • Microarchitecture
  • Osteoporosis
  • Prenatal alcohol
  • Trabeculae

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