Isolation and preliminary characterization of a caprine rotavirus.

V. M. Mendes; M. C. De Beer; G. H. Goosen; A. D. Steele

Isolation and preliminary characterization of a caprine rotavirus.

V. M. Mendes^*, M. C. De Beer, G. H. Goosen, A. D. Steele

^*Corresponding author for this work

Virological Pathology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Five cytopathic rotavirus strains were isolated in MA104 cells from stool specimens of kids with diarrhoea. Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance medium were important for the successful cultivation of the strains in these cells. The isolates were shown to be group A rotaviruses by antigenic reactivity with a group A monoclonal antibody. This was confirmed by the migration patterns of the viral RNA genome during polyacrylamide gel electrophoresis, which also confirmed that all five strains had an identical RNA electropherotype. Analysis with monoclonal antibodies to the subgroup-specific VP6 antigen showed that these strains carried the subgroup I epitope.

Original language	English
Pages (from-to)	291-294
Number of pages	4
Journal	The Onderstepoort journal of veterinary research
Volume	61
Issue number	4
Publication status	Published - Dec 1994

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title = "Isolation and preliminary characterization of a caprine rotavirus.",

abstract = "Five cytopathic rotavirus strains were isolated in MA104 cells from stool specimens of kids with diarrhoea. Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance medium were important for the successful cultivation of the strains in these cells. The isolates were shown to be group A rotaviruses by antigenic reactivity with a group A monoclonal antibody. This was confirmed by the migration patterns of the viral RNA genome during polyacrylamide gel electrophoresis, which also confirmed that all five strains had an identical RNA electropherotype. Analysis with monoclonal antibodies to the subgroup-specific VP6 antigen showed that these strains carried the subgroup I epitope.",

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AU - De Beer, M. C.

AU - Goosen, G. H.

AU - Steele, A. D.

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AB - Five cytopathic rotavirus strains were isolated in MA104 cells from stool specimens of kids with diarrhoea. Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance medium were important for the successful cultivation of the strains in these cells. The isolates were shown to be group A rotaviruses by antigenic reactivity with a group A monoclonal antibody. This was confirmed by the migration patterns of the viral RNA genome during polyacrylamide gel electrophoresis, which also confirmed that all five strains had an identical RNA electropherotype. Analysis with monoclonal antibodies to the subgroup-specific VP6 antigen showed that these strains carried the subgroup I epitope.

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JF - The Onderstepoort journal of veterinary research

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ER -

Isolation and preliminary characterization of a caprine rotavirus.

Abstract

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