Isolation of a mixture of Phytosterol compounds from the n-Hexane extract of Jatropha lagarinthoides (Sond) collected from Zebediela sub-region in Limpopo province, South Africa

Sechene Stanley Gololo*, Leshwene Jeremiah Shai, Lesibana Sethoga, Nana Agyei, Kikoete Edward Bassey, Motetelo Alfred Mogale

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The study aimed to isolate and identify the active principles from the leaves of Jatropha lagarinthoides. The dried leaves powder of Jatropha lagarinthoides was extracted with n-Hexane by cold maceration and subjected to silica gel 60 column chromatography that afforded isolation of a fraction (CPD1JH) with free radical scavenging activity and Rf value of 0.54. Mass spectrum of CPD1JH showed a molecular ion peak at m/z 412 which correspond to molecular formula C29H48O. 1H-NMR (acetone) spectrum of CPD1JH showed a multiplet signal at δ 3.36, 3.31 and an olefinic proton at δ 5.37. Other olefinic protons appeared as multiplets at δ 5.17, 4.12 and δ 406. Methyl protons appeared at δ 1.03, 0.89, 0.85 - 0.82. The isolated fraction also tested positive for both steroid and alcohol. Based on the chemical and spectroscopic characteristics of the isolated fraction, CPD1JH was concluded to be a mixture of two phytosterol compounds, Stigmasterol and β-Sitosterol.

Original languageEnglish
Pages (from-to)3084-3087
Number of pages4
JournalJournal of Chemical and Pharmaceutical Sciences
Volume9
Issue number4
Publication statusPublished - 2016

Keywords

  • ?-sitosterol
  • Cold maceration
  • Jatropha lagarinthoides
  • N-hexane extract
  • Silica gel 60 column chromatography
  • Stigmasterol

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