The study aimed to isolate and identify the active principles from the leaves of Jatropha lagarinthoides. The dried leaves powder of Jatropha lagarinthoides was extracted with n-Hexane by cold maceration and subjected to silica gel 60 column chromatography that afforded isolation of a fraction (CPD1JH) with free radical scavenging activity and Rf value of 0.54. Mass spectrum of CPD1JH showed a molecular ion peak at m/z 412 which correspond to molecular formula C29H48O. 1H-NMR (acetone) spectrum of CPD1JH showed a multiplet signal at δ 3.36, 3.31 and an olefinic proton at δ 5.37. Other olefinic protons appeared as multiplets at δ 5.17, 4.12 and δ 406. Methyl protons appeared at δ 1.03, 0.89, 0.85 - 0.82. The isolated fraction also tested positive for both steroid and alcohol. Based on the chemical and spectroscopic characteristics of the isolated fraction, CPD1JH was concluded to be a mixture of two phytosterol compounds, Stigmasterol and β-Sitosterol.
|Number of pages||4|
|Journal||Journal of Chemical and Pharmaceutical Sciences|
|Publication status||Published - 2016|
- Cold maceration
- Jatropha lagarinthoides
- N-hexane extract
- Silica gel 60 column chromatography