Isolation of an Iridoid glycoside compound from the leaves of Barleria dinteri collected from Zebediela sub-region in Limpopo province, South Africa

S. S. Gololo*, K. Bassey, M. T. Olivier, N. M. Agyei, L. J. Shai, P. Masoko, M. Gamedze, M. A. Mogale

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Barleria dinteri (Acanthaceae) is a perennial shrub that is widely distributed in Southern Africa. The leaves of the plant species are used in traditional medicine by South African healers to promote the healing of wounds, treatment of some intestinal tumours, infectious diseases and to relieve joint pains and toothache. The aim of the study was to isolate and identify the active ingredient(s) from the leaves of the South African B. dinteri species that contribute to the medicinal importance of the plant species. The ground leaves of B. dinteri were extracted with hexane, dichloromethane, acetone and methanol through a serial exhaustive extraction procedure. The obtained extracts were screened for free radical scavenging activity using diphenyl-1-picrylhydrazil (DPPH) bioassay. Furthermore, the methanol extract was fractionated using silica gel 60 column chromatography and the purified fraction was characterized using NMR. All the leaf extracts of B. dinteri indicated potential for free radical scavenging. A fraction suggesting to be representing a compound in pure form upon TLC analysis was obtained by the fractionation of the methanol extract using gel 60 column chromatography. Following NMR characterization of the purified fraction, the obtained compound was identified as an iridoid glycoside, Barledinoside. The isolation and identification of barlerin from the leaves of Barleria dinteri justifies the usage of the plant species in traditional medicine by local communities of Zebediela sub-region in Limpopo province, South Africa.

Original languageEnglish
Pages (from-to)1368-1372
Number of pages5
JournalJournal of Pharmaceutical Sciences and Research
Volume9
Issue number8
Publication statusPublished - Aug 2017

Keywords

  • Barleria dinteri
  • Fractionation
  • Free radical scavenging activity
  • Iridoid glycoside
  • Isolation
  • NMR

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