TY - JOUR
T1 - Isoniazid acetylation phenotypes in the Sudanese population; findings and implications
AU - Ali, Monadil H.
AU - Alrasheedy, Alian A.
AU - Kibuule, Dan
AU - Hassali, Mohamed Azmi
AU - Godman, Brian
AU - Abdelwahab, Mohammed F.
AU - Abbadi, Raef Y.
N1 - Funding Information:
The authors acknowledge the staff of Northern Border University, Saudi Arabia who facilitated the processing of samples and instrumental analysis. Special thank you also goes to laboratory department and Pharmacy section, Rafha Central Hospital for the provision of isoniazid and heparinized tubes and other consumables. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. This is to confirm that the study received ethical approval from the Committee of Bioethics at Northern Border University, Saudi Arabia with decision number (4/38/H) and dated 5/01/2017. The purpose of the study was clearly explained to the participants and written consents were then taken.
Publisher Copyright:
© 2019 The Author(s)
PY - 2019/12
Y1 - 2019/12
N2 - Background: Isoniazid (INH) is the mainstay antimicrobial in the treatment of tuberculosis (TB). It is acetlylated in the liver to acetyl-INH. However, there is variation in rate of acetylation of INH among TB patients (i.e. fast, intermediate or slow acetylators) which impacts on the treatment outcomes. Aim: The isoniazid acetylation phenotypes in the expatriate Sudanese population were determined to provide future guidance since TB is prevalent in Sudan. Methods: A community-based trial among Sudanese expatriates in Saudi Arabia was undertaken to identify INH-acetylation phenotypes. After overnight fasting, a single dose of 200 mg of INH was given to the volunteers. Three hours later, 5 ml of blood were drawn from each volunteer and prepared for High-Performance Liquid Chromatography (HPLC) analysis. The main outcomes were INH and Acetyl-INH concentrations in plasma and the subsequent Acetyl-INH/INH metabolic ratio (MR). Results: The findings suggest that slow acetylation is highly prevalent among the study participants (n = 43; 84.31%). Moreover, there was no statistically significant correlation between age and the MR (r = −0.18, P = 0.20). Further, there was no significant association between gender and the MR (P = 0.124). Similarly, no significant association was found between smoking habits and MR (P = 0.24). Conclusion: Isoniazid phenotyping suggests predominantly slow acetylation among the Sudanese in this sample. The study found no statistically significant associations between the MR and age or gender or smoking.
AB - Background: Isoniazid (INH) is the mainstay antimicrobial in the treatment of tuberculosis (TB). It is acetlylated in the liver to acetyl-INH. However, there is variation in rate of acetylation of INH among TB patients (i.e. fast, intermediate or slow acetylators) which impacts on the treatment outcomes. Aim: The isoniazid acetylation phenotypes in the expatriate Sudanese population were determined to provide future guidance since TB is prevalent in Sudan. Methods: A community-based trial among Sudanese expatriates in Saudi Arabia was undertaken to identify INH-acetylation phenotypes. After overnight fasting, a single dose of 200 mg of INH was given to the volunteers. Three hours later, 5 ml of blood were drawn from each volunteer and prepared for High-Performance Liquid Chromatography (HPLC) analysis. The main outcomes were INH and Acetyl-INH concentrations in plasma and the subsequent Acetyl-INH/INH metabolic ratio (MR). Results: The findings suggest that slow acetylation is highly prevalent among the study participants (n = 43; 84.31%). Moreover, there was no statistically significant correlation between age and the MR (r = −0.18, P = 0.20). Further, there was no significant association between gender and the MR (P = 0.124). Similarly, no significant association was found between smoking habits and MR (P = 0.24). Conclusion: Isoniazid phenotyping suggests predominantly slow acetylation among the Sudanese in this sample. The study found no statistically significant associations between the MR and age or gender or smoking.
KW - Acetylation
KW - Fast acetylators
KW - INH
KW - Isoniazid
KW - Slow acetylators
KW - Sudanese populations
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=85072232624&partnerID=8YFLogxK
U2 - 10.1016/j.jctube.2019.100120
DO - 10.1016/j.jctube.2019.100120
M3 - Article
AN - SCOPUS:85072232624
SN - 2405-5794
VL - 17
JO - Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
JF - Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
M1 - 100120
ER -