TY - JOUR
T1 - Molecular characterization and genotyping of human rotavirus strains in abidjan, cote d'ivoire
AU - Akran, Veronique
AU - Peenze, I.
AU - Akoua-Koffi, C.
AU - Kette, H.
AU - De Beer, M. C.
AU - Dosso, M.
AU - Steele, A. D.
N1 - Funding Information:
Financial support: Department of Immunization and Biologicals, World Health Organization (research grant V27/181/113), South African Medical Research Council, Tygerberg (travel support), and Poliomyelitis Research Foundation, Sandringham, South Africa (travel support).
Funding Information:
Supplement sponsorship: This article is part of a supplement entitled “Rotavirus Infection In Africa: Epidemiology, Burden of Disease, and Strain Diversity,” which was prepared as a project of the Rotavirus Vaccine Program, a partnership among PATH, the World Health Organization, and the US Centers for Disease Control and Prevention, and was funded in full or in part by the GAVI Alliance.
PY - 2010/9/1
Y1 - 2010/9/1
N2 - In this study, we characterized human rotavirus strains recovered from infants and young children with acute diarrhea in Abidjan, Cote d'Ivoire, during 2000-2004. In total, 719 fecal specimens were collected from children aged 1-60 months with acute infantile gastroenteritis. Examination with a commercial enzyme-linked immunosorbent assay showed the presence of group A rotavirus antigen in 208 diarrheal specimens (28.9%). Polyacrylamide gel electrophoresis of the RNA extracted from rotavirus-positive stools yielded a variety of "long" and "short" RNA electropherotypes, which were used to help select strains for VP4 and VP7 genotyping. VP7 genotype G1 strains were circulating most commonly during the study period (53%), followed by G2 (22%) and G3 (5%) strains. Strains with multiple VP7 genotype reactivity were observed in 7.6% of specimens, and a similar number (8%) could not be typed at all. VP4 P[6] and P[8] strains circulated at similar levels (33%). Strains demonstrating multiple VP4 types were quite common (9%); however, 20% of the strains were untypeable by the methods used. Rotavirus strain diversity in Cote d'Ivoire was similar to that observed in other West African countries.
AB - In this study, we characterized human rotavirus strains recovered from infants and young children with acute diarrhea in Abidjan, Cote d'Ivoire, during 2000-2004. In total, 719 fecal specimens were collected from children aged 1-60 months with acute infantile gastroenteritis. Examination with a commercial enzyme-linked immunosorbent assay showed the presence of group A rotavirus antigen in 208 diarrheal specimens (28.9%). Polyacrylamide gel electrophoresis of the RNA extracted from rotavirus-positive stools yielded a variety of "long" and "short" RNA electropherotypes, which were used to help select strains for VP4 and VP7 genotyping. VP7 genotype G1 strains were circulating most commonly during the study period (53%), followed by G2 (22%) and G3 (5%) strains. Strains with multiple VP7 genotype reactivity were observed in 7.6% of specimens, and a similar number (8%) could not be typed at all. VP4 P[6] and P[8] strains circulated at similar levels (33%). Strains demonstrating multiple VP4 types were quite common (9%); however, 20% of the strains were untypeable by the methods used. Rotavirus strain diversity in Cote d'Ivoire was similar to that observed in other West African countries.
UR - http://www.scopus.com/inward/record.url?scp=77955680307&partnerID=8YFLogxK
U2 - 10.1086/653567
DO - 10.1086/653567
M3 - Article
C2 - 20684706
AN - SCOPUS:77955680307
SN - 0022-1899
VL - 202
SP - S220-S224
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - SUPPL. 1
ER -
