TY - JOUR
T1 - Novel Mycobacterium avium Complex Species Isolated From Black Wildebeest (Connochaetes gnou) in South Africa
AU - Kabongo-Kayoka, P. N.
AU - Obi, C. L.
AU - Nakajima, C.
AU - Suzuki, Y.
AU - Hattori, T.
AU - Eloff, J. N.
AU - Wright, J.
AU - Mbelle, N.
AU - McGaw, L. J.
N1 - Funding Information:
The research protocol was approved by the Ethical Research Committee at University of South Africa (UNISA) Reference number: CAES/087; the National Research Foundation (NRF) unique numbers: 66141 and 86458, and the Department of Agriculture, Forestry and Fisheries (DAFF). This research study was supported by NRF under the aegis of the bilateral research collaboration between South Africa and the Japan Society for the Promotion of Science (JSPS). Financial support was also provided by the University of South Africa. We would like to thank DAFF for approving this research project and National Health Laboratory Service for making the facilities available. The state veterinarians namely Dr Jacoba Wessels and Dr Melissa Nel (nee Hansen) are immensely thanked for providing the samples as well as the history and histopathological results. We acknowledge the technical assistance of the following people: Yukari Fukujima and Haruka Suzuki from the Research Center for Zoonosis Control at Hokkaido University in Japan; and Kathy Lindeque, Buki Onwuebuna and Charlotte Kekana from National Health Laboratory Service South Africa.
Publisher Copyright:
© 2015 Blackwell Verlag GmbH
PY - 2017/6
Y1 - 2017/6
N2 - A study was undertaken to isolate and characterize Mycobacterium species from black wildebeest suspected of being infected with tuberculosis in South Africa. This led to the discovery of a new Mycobacterium avium complex species, provisionally referred to as the Gnou isolate from black wildebeest (Connochaetes gnou). Sixteen samples from nine black wildebeest were processed for Mycobacterium isolation. Following decontamination, samples were incubated in an ordinary incubator at 37°C on Löwenstein–Jensen slants and in liquid medium tubes using the BACTEC™ MGIT™ 960 system, respectively. Identification of the isolate was carried out by standard biochemical tests and using the line probe assay from the GenoType® CM/AS kit (Hain Lifescience GmbH, Nehren, Germany). The DNA extract was also analysed using gene sequencing. Partial gene sequencing and analysis of 16S rRNA gene, and 16S-23S rRNA (ITS), rpoB and hsp65 and phylogenetic analyses by searching GenBank using the BLAST algorithm were conducted. Phylogenetic trees were constructed using four methods, namely Bayesian inference, maximum likelihood, maximum parsimony and neighbour-joining methods. The isolate was identified as Mycobacterium intracellulare using the GenoType® CM/AS kit and as Mycobacterium avium complex (MAC) by gene sequencing. The gene sequence targeting all the genes, ITS, 16S rRNA, rpoB and hsp65 and phylogenetic analyses indicated that this isolate presented a nucleotide sequence different from all currently published sequences, and its position was far enough from other MAC species to suggest that it might be a new species.
AB - A study was undertaken to isolate and characterize Mycobacterium species from black wildebeest suspected of being infected with tuberculosis in South Africa. This led to the discovery of a new Mycobacterium avium complex species, provisionally referred to as the Gnou isolate from black wildebeest (Connochaetes gnou). Sixteen samples from nine black wildebeest were processed for Mycobacterium isolation. Following decontamination, samples were incubated in an ordinary incubator at 37°C on Löwenstein–Jensen slants and in liquid medium tubes using the BACTEC™ MGIT™ 960 system, respectively. Identification of the isolate was carried out by standard biochemical tests and using the line probe assay from the GenoType® CM/AS kit (Hain Lifescience GmbH, Nehren, Germany). The DNA extract was also analysed using gene sequencing. Partial gene sequencing and analysis of 16S rRNA gene, and 16S-23S rRNA (ITS), rpoB and hsp65 and phylogenetic analyses by searching GenBank using the BLAST algorithm were conducted. Phylogenetic trees were constructed using four methods, namely Bayesian inference, maximum likelihood, maximum parsimony and neighbour-joining methods. The isolate was identified as Mycobacterium intracellulare using the GenoType® CM/AS kit and as Mycobacterium avium complex (MAC) by gene sequencing. The gene sequence targeting all the genes, ITS, 16S rRNA, rpoB and hsp65 and phylogenetic analyses indicated that this isolate presented a nucleotide sequence different from all currently published sequences, and its position was far enough from other MAC species to suggest that it might be a new species.
KW - M. intracellulare
KW - Mycobacterium avium complex
KW - black wildebeest
KW - non-tuberculous mycobacterium
KW - phylogenetic analyses
UR - http://www.scopus.com/inward/record.url?scp=84951091329&partnerID=8YFLogxK
U2 - 10.1111/tbed.12460
DO - 10.1111/tbed.12460
M3 - Article
C2 - 26671520
AN - SCOPUS:84951091329
SN - 1865-1674
VL - 64
SP - 929
EP - 937
JO - Transboundary and Emerging Diseases
JF - Transboundary and Emerging Diseases
IS - 3
ER -