Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption

Marianne Haag*, Olga N. Magada, Nico Claassen, Linde H. Böhmer, Marlena C. Kruger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)


Dietary supplementation with fish oil that contains omega-3 polyunsaturated fatty acids has been shown to enhance bone density as well as duodenal calcium uptake in rats. The latter process is supported by membrane ATPases. The present in vitro study was undertaken to test the effect of omega-3 fatty acids on ATPase activity in isolated basolateral membranes from rat duodenal enterocytes. Ca-ATPase in calmodulin-stripped membranes was activated in a biphasic manner by docosahexanoic acid (DHA) (10-30 μg/ml) but not by eicosapentanoic acid (EPA). This effect was blocked partially by 0.5 μM calphostin (a protein kinase C blocker). DHA inhibited Na,K-ATPase (-49% of basal activity, [DHA] = 30 μg/ml, P<0.01). This effect could be reversed partially by 50 μM genistein, a tyrosine kinase blocker. EPA also inhibited Na,K-ATPase: (-47% of basal activity, [EPA]=30 μg/ml, P<0.01), this effect was partially reversed by 100 μM indomethacin, a cyclo-oxygenase blocker. Omega-3 fatty acids are thus involved in multiple signalling effects that effect ATPases in BLM.

Original languageEnglish
Pages (from-to)423-429
Number of pages7
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Issue number6
Publication statusPublished - 1 Jun 2003


  • Ca absorption
  • Ca-ATPase
  • Cellular signalling
  • Docosahexanoic acid
  • Duodenum
  • Eicosapentanoic acid
  • Na,K-ATPase
  • Omega-3 fatty acids
  • Polyunsaturated fatty acids


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