Phylogenetic studies of larval digenean trematodes from freshwater snails and fish species in the proximity of Tshwane Metropolitan, South Africa

Esmey B. Moema*, Pieter H. King, Johnny N. Rakgole

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The classification and description of digenean trematodes are commonly accomplished by using morphological features, especially in adult stages. The aim of this study was to provide an analysis of the genetic composition of larval digenean trematodes using polymerase chain reaction (PCR) and sequence analysis. Deoxyribonucleic acid (DNA) was extracted from clinostomatid metacercaria, 27-spined echinostomatid redia, avian schistosome cercaria and strigeid metacercaria from various dams in the proximity of Tshwane metropolitan, South Africa. Polymerase chain reaction was performed using the extracted DNA with primers targeting various regions within the larval digenean trematodes’ genomes. Agarose gel electrophoresis technique was used to visualise the PCR products. The PCR products were sequenced on an Applied Bioinformatics (ABI) genetic analyser platform. Genetic information obtained from this study had a higher degree of discrimination than the morphological characteristics of seemingly similar organisms.

Original languageEnglish
Article numbera1726
JournalOnderstepoort Journal of Veterinary Research
Volume86
Issue number1
DOIs
Publication statusPublished - 2019

Keywords

  • Classification
  • Description
  • Digenean trematodes
  • Genetic composition
  • Molecular analysis
  • Nucleotide variations
  • PCR
  • Polymerase chain reaction
  • Sequence analysis

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