TY - JOUR
T1 - Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)
AU - Fasina, Folorunso O.
AU - Olaokun, Oyinlola O.
AU - Oladipo, Olusola O.
AU - Fasina, Margaret M.
AU - Makinde, Adesoji A.
AU - Heath, Livio
AU - Bastos, Armanda D.S.
N1 - Funding Information:
The plant Ancistrocladus uncinatus,was identified by Mal. U. S. Gallah of the Ahmadu Bello University, Zaria, Nigeria with a Voucher Number. 109413, (Date: 9/4/13). We wish to thank Ms Dipolelo Semenya and Nontobeko Mtshali & Dr. Juanita van Heerden for assistance with supplies of primary cell cultures and molecular biology respectively; and Ms Selaelo Raphatlelo for the TLC. Members of the ASF Project Implementation Task Team (ASF PITT) of the National Veterinary Research Institute, Vom, Nigeria are thanked for their inputs. Special thanks to Prof. J. N. Eloff for permitting the use of his lab for certain aspects of the work, The Executive Secretary, Agricultural Research Council of Nigeria, Abuja for part funding of this project under the Project Code 025060410100000 (Development of rapid and effective Diagnostic and Control tools for African Swine Fever) and the Executive Director, National Veterinary Research Institute, Dr. M. S. Ahmed for his support. Prof. Louis Maes of the Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp is thanked for supplying the excel worksheet for calculating the IC50 for the plant extract.
PY - 2013/6/19
Y1 - 2013/6/19
N2 - Background: African swine fever (ASF), a highly contagious fatal acute haemorrhagic viral disease of pigs currently has no treatment or vaccination protocol and it threatens the pig industry worldwide. Recent outbreaks were managed by farmers with ethnoveterinary preparations with various claims of effectiveness.Results: We identified 35 compounds using GC-MS protocol and ASF virus (NIG 99) was significantly reduced by some extracts and fractions of the plant. However, the plant was poorly extracted by water and cytotoxicity was found to be a major problem with the use of the plant since its extracts also reduced the primary cells used in the assay.Conclusion: It is confirmed that the plant has antiviral potentials against ASF virus and farmers' claims seem to have certain degree of veracity, but finding the best means of exploring the potential of the plant while reducing its cytotoxic effect in-vitro and in-vivo will be necessary.
AB - Background: African swine fever (ASF), a highly contagious fatal acute haemorrhagic viral disease of pigs currently has no treatment or vaccination protocol and it threatens the pig industry worldwide. Recent outbreaks were managed by farmers with ethnoveterinary preparations with various claims of effectiveness.Results: We identified 35 compounds using GC-MS protocol and ASF virus (NIG 99) was significantly reduced by some extracts and fractions of the plant. However, the plant was poorly extracted by water and cytotoxicity was found to be a major problem with the use of the plant since its extracts also reduced the primary cells used in the assay.Conclusion: It is confirmed that the plant has antiviral potentials against ASF virus and farmers' claims seem to have certain degree of veracity, but finding the best means of exploring the potential of the plant while reducing its cytotoxic effect in-vitro and in-vivo will be necessary.
KW - African swine fever virus
KW - Ancistrocladus uncinatus
KW - Antiviral
UR - http://www.scopus.com/inward/record.url?scp=84879046278&partnerID=8YFLogxK
U2 - 10.1186/1746-6148-9-120
DO - 10.1186/1746-6148-9-120
M3 - Article
C2 - 23777548
AN - SCOPUS:84879046278
SN - 1746-6148
VL - 9
JO - BMC Veterinary Research
JF - BMC Veterinary Research
M1 - 120
ER -