TY - JOUR
T1 - Phytochemical, anti-inflammatory and anti-trypanosomal properties of Anthocleista vogelii Planch (Loganiaceae) stem bark
AU - Eze, Fabian Ifeanyi
AU - Siwe Noundou, Xavier
AU - Osadebe, Patience O.
AU - Krause, Rui W.M.
N1 - Funding Information:
This work was sponsored by the Nigeria’s Tertiary Education Trust Fund (TETFund) under the TETfund Institution-Based Research (IBR) Intervention and supported by the South African Medical Research Council (MRC) with funds from National Treasury under its Economic Competitiveness and Support Package, and Rhodes University SandisaImbewu. The authors are grateful to Michelle Isaac for assistance in the bioassays. X. Siwe Noundou is grateful for a Rhodes University Postdoctoral Research Fellowship.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/6/28
Y1 - 2019/6/28
N2 - Ethnopharmacological relevance: Anthocleista vogelii Planch (Loganiaceae) is used in African Traditional Medicine for the treatment of pain and inflammatory disorders as well as sleeping sickness. Aim of the study: To determine the in vivo anti-inflammatory and in vitro anti-trypanosomal activities of the extracts of A. vogelii stem bark and identify the phytochemical classes of the fractions responsible for the activities. Materials and methods: The in vivo anti-inflammatory activity of the extracts was evaluated using the egg albumin-induced rat paw oedema model while the in vitro anti-trypanosomal activity was assessed on Trypanosoma brucei brucei. The in vitro cytotoxicity was assessed on HeLa (human cervix adenocarcinoma) cell line. Results: The methanolic extract of A. vogelii stem bark, with 11.2% yield, gave LD 50 > 5000 mg/kg. The n-hexane fraction of the extract contains steroids, terpenes and fatty acids and yielded non-cytotoxic terpenoidal column fraction with anti-trypanosomal IC 50 of 3.0 μg/mL. The ethylacetate fraction at 100 mg/kg dose significantly (p < 0.05) provoked 37.8, 62.5 and 69.7% inhibition of oedema induced by egg-albumin at the second, fourth and sixth hours respectively. Conclusion: The study demonstrated that the anti-inflammatory and anti-trypanosomal activities of A. vogelii are probably due to non-cytotoxic terpenoids and validated the traditional use of A. vogelii in the treatment of inflammation and sleeping sickness.
AB - Ethnopharmacological relevance: Anthocleista vogelii Planch (Loganiaceae) is used in African Traditional Medicine for the treatment of pain and inflammatory disorders as well as sleeping sickness. Aim of the study: To determine the in vivo anti-inflammatory and in vitro anti-trypanosomal activities of the extracts of A. vogelii stem bark and identify the phytochemical classes of the fractions responsible for the activities. Materials and methods: The in vivo anti-inflammatory activity of the extracts was evaluated using the egg albumin-induced rat paw oedema model while the in vitro anti-trypanosomal activity was assessed on Trypanosoma brucei brucei. The in vitro cytotoxicity was assessed on HeLa (human cervix adenocarcinoma) cell line. Results: The methanolic extract of A. vogelii stem bark, with 11.2% yield, gave LD 50 > 5000 mg/kg. The n-hexane fraction of the extract contains steroids, terpenes and fatty acids and yielded non-cytotoxic terpenoidal column fraction with anti-trypanosomal IC 50 of 3.0 μg/mL. The ethylacetate fraction at 100 mg/kg dose significantly (p < 0.05) provoked 37.8, 62.5 and 69.7% inhibition of oedema induced by egg-albumin at the second, fourth and sixth hours respectively. Conclusion: The study demonstrated that the anti-inflammatory and anti-trypanosomal activities of A. vogelii are probably due to non-cytotoxic terpenoids and validated the traditional use of A. vogelii in the treatment of inflammation and sleeping sickness.
KW - Anthocleista vogelii
KW - Anti-inflammatory
KW - Anti-trypanosomal
KW - Cytotoxicity
KW - Phytochemical analysis
UR - http://www.scopus.com/inward/record.url?scp=85064190961&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2019.111851
DO - 10.1016/j.jep.2019.111851
M3 - Article
C2 - 30978458
AN - SCOPUS:85064190961
SN - 0378-8741
VL - 238
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
M1 - 111851
ER -