TY - JOUR
T1 - Phytochemical screening, antioxidant and antibacterial activities of ethanol extracts of Asparagus suaveolens aerial parts
AU - Olivier, Mutendela Tabile
AU - Muganza, F. M.
AU - Shai, L. J.
AU - Gololo, Sechene Stanley
AU - Nemutavhanani, L. D.
N1 - Funding Information:
We are very much thankful to Sefako Makgatho Health Sciences University for financial assistance, and the NHLS laboratories (Johannesburg and Ga-Rankuwa) for providing us with the infrastructures as well as the microorganisms used in this study. Our gratitude is also extended to the HOD (Professor Nana Agyei) of Chemistry Department at Sefako Makgatho Health Sciences University for his continuous support.
Publisher Copyright:
© 2016 SAAB
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - © 2016 SAAB This study was aimed at investigating the phytochemical constituents, antioxidant activities and antibacterial activities of Asparagus suaveolens (A. suaveolens) aerial parts. Antioxidant properties were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, hydrogen peroxide scavenging assay and reducing power assay. Antibacterial activity of the extract was assessed by micro-dilution method and 2008 WHO Neisseria gonorrhoea (N. gonorrhoea) reference strains were used for this purpose. Qualitative phytochemical analysis showed the presence of alkaloids, saponins, flavonoids, glycosides, anthraquinones, terpenoids and steroids, reducing sugar, proteins and coumarins. An effective free radical scavenging activity of 71% was observed for chloroform fraction at 2.5 mg/mL. Butan-1-ol fraction had 79% inhibition of hydrogen peroxide and 55% reducing power at 2.0 mg/mL compared to all other fractions but less than the standards. The n-hexane fraction had moderate growth inhibition against F, O and P 2008 WHO N. gonorrhoea reference strains with MIC values of 1.3 mg/mL, 0.12 mg/mL and 1.63 mg/mL, respectively. The MBC values of the same fraction had moderate activities against the same strains. This promising anti-N. gonorrhoea properties of A. suaveolens aerial parts observed confirms its traditional use in the treatment of gonorrhoea infections by Bolahlakgomo Village commuters of the Limpopo Province, South Africa.
AB - © 2016 SAAB This study was aimed at investigating the phytochemical constituents, antioxidant activities and antibacterial activities of Asparagus suaveolens (A. suaveolens) aerial parts. Antioxidant properties were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, hydrogen peroxide scavenging assay and reducing power assay. Antibacterial activity of the extract was assessed by micro-dilution method and 2008 WHO Neisseria gonorrhoea (N. gonorrhoea) reference strains were used for this purpose. Qualitative phytochemical analysis showed the presence of alkaloids, saponins, flavonoids, glycosides, anthraquinones, terpenoids and steroids, reducing sugar, proteins and coumarins. An effective free radical scavenging activity of 71% was observed for chloroform fraction at 2.5 mg/mL. Butan-1-ol fraction had 79% inhibition of hydrogen peroxide and 55% reducing power at 2.0 mg/mL compared to all other fractions but less than the standards. The n-hexane fraction had moderate growth inhibition against F, O and P 2008 WHO N. gonorrhoea reference strains with MIC values of 1.3 mg/mL, 0.12 mg/mL and 1.63 mg/mL, respectively. The MBC values of the same fraction had moderate activities against the same strains. This promising anti-N. gonorrhoea properties of A. suaveolens aerial parts observed confirms its traditional use in the treatment of gonorrhoea infections by Bolahlakgomo Village commuters of the Limpopo Province, South Africa.
KW - Antibacterial activity
KW - Antioxidant activity
KW - Asparagus suaveolens
KW - Minimum inhibitory concentration
UR - http://www.scopus.com/inward/record.url?scp=84989311683&partnerID=8YFLogxK
U2 - 10.1016/j.sajb.2016.09.014
DO - 10.1016/j.sajb.2016.09.014
M3 - Article
SN - 0254-6299
VL - 108
SP - 41
EP - 46
JO - South African Journal of Botany
JF - South African Journal of Botany
ER -
