Predominance of rotavirus G1[P8] genotype among under-five children with gastroenteritis in Mwanza, Tanzania

Adolfine Hokororo, Benson R Kidenya, Jeremiah Seni, Seheri Mapaseka, Jeffrey Mphahlele, Stephen E Mshana

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We analyzed stool samples from underfives with gastroenteritis for rotavirus infection between January 2010 and June 2011. A total of 393 stool specimens were examined for rotavirus infection using enzyme-linked immunosorbent assay (ELISA). Hundred selected positive specimens were genotyped using multiplex polymerase chain reaction. Out of 393 underfives, 194 (49.4%) had rotavirus infection, with 96.9% of infected underfives being <2 years. Underfives infected with rotavirus had prolonged hospital stay than those without rotavirus infection (P = 0.0001). G1 was the most predominant G type (59%) followed by G8 (13%) while P[8] was the most predominant P type (25%). In single-type infection, common G-P combinations were G1P[8] (24%) and G1P[6] (17%). Common mixed infections were G1/G8 (16%) and P4/P8 (13%). G1 genotype is common among underfives with gastroenteritis in Mwanza. Diversity of genotypes causing gastroenteritis in Mwanza necessitates a continuous surveillance after the introduction of RotaRix® vaccine.

Original languageEnglish
Pages (from-to)393-6
Number of pages4
JournalJournal of Tropical Pediatrics
Volume60
Issue number5
DOIs
Publication statusPublished - Oct 2014

Keywords

  • Child, Preschool
  • Cross-Sectional Studies
  • Enzyme-Linked Immunosorbent Assay
  • Feces/virology
  • Female
  • Gastroenteritis/epidemiology
  • Genotype
  • Humans
  • Infant
  • Male
  • Multiplex Polymerase Chain Reaction
  • Prospective Studies
  • RNA, Viral/genetics
  • Rotavirus/classification
  • Rotavirus Infections/epidemiology
  • Tanzania/epidemiology

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