Abstract
We analyzed stool samples from underfives with gastroenteritis for rotavirus infection between January 2010 and June 2011. A total of 393 stool specimens were examined for rotavirus infection using enzyme-linked immunosorbent assay (ELISA). Hundred selected positive specimens were genotyped using multiplex polymerase chain reaction. Out of 393 underfives, 194 (49.4%) had rotavirus infection, with 96.9% of infected underfives being <2 years. Underfives infected with rotavirus had prolonged hospital stay than those without rotavirus infection (P = 0.0001). G1 was the most predominant G type (59%) followed by G8 (13%) while P[8] was the most predominant P type (25%). In single-type infection, common G-P combinations were G1P[8] (24%) and G1P[6] (17%). Common mixed infections were G1/G8 (16%) and P4/P8 (13%). G1 genotype is common among underfives with gastroenteritis in Mwanza. Diversity of genotypes causing gastroenteritis in Mwanza necessitates a continuous surveillance after the introduction of RotaRix® vaccine.
Original language | English |
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Pages (from-to) | 393-6 |
Number of pages | 4 |
Journal | Journal of Tropical Pediatrics |
Volume | 60 |
Issue number | 5 |
DOIs | |
Publication status | Published - Oct 2014 |
Keywords
- Child, Preschool
- Cross-Sectional Studies
- Enzyme-Linked Immunosorbent Assay
- Feces/virology
- Female
- Gastroenteritis/epidemiology
- Genotype
- Humans
- Infant
- Male
- Multiplex Polymerase Chain Reaction
- Prospective Studies
- RNA, Viral/genetics
- Rotavirus/classification
- Rotavirus Infections/epidemiology
- Tanzania/epidemiology