Prevalence of Campylobacter species, Helicobacter pylori and Arcobacter species in stool samples from the Venda region, Limpopo, South Africa: Studies using molecular diagnostic methods

A. Samie*, C. L. Obi, L. J. Barrett, S. M. Powell, R. L. Guerrant

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

97 Citations (Scopus)

Abstract

Objectives: This study determined the prevalence of Campylobacter spp., Helicobacter pylori and Arcobacter spp. in stool samples from Venda in relation to diarrhea, intestinal inflammation and HIV status using specific molecular methods. Methods: Stool samples were collected from hospital patients (255) and primary school children (67). Genomic DNA was extracted from the stools and molecular methods including PCR, PCR followed by restriction analysis and multiplex PCR were used to test for the different organisms. The lactoferrin content of the stools was determined using commercial kits from TechLab (Blacksburg, VA, USA). Results: The prevalence of the different organisms was 50.6% for H. pylori, 10.2% for C. jejuni, 6.2% for A. butzleri, 6.5% for C. coli, 3.1% for C. concisus, 2.8% for A. cryaerophilus and 1.9% for A. skirrowii. Of all the organisms, only C. jejuni was significantly associated with diarrhea (84.8%) (χ2 = 21.025, P < 0.001) and elevated levels of lactoferrin (78.8%) (χ2 = 16.919, P < 0.005) and was an important pathogen associated with diarrhea among HIV positive individuals (22.8%). Conclusions: Campylobacter infections are common causes of gastroenteritis in Venda. Non-C. jejuni/coli Campylobacters such as C. concisus as well as A. butzleri and H. pylori may be involved in gastrointestinal diseases in the region but further studies are needed to confirm this hypothesis.

Original languageEnglish
Pages (from-to)558-566
Number of pages9
JournalJournal of Infection
Volume54
Issue number6
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Keywords

  • Arcobacter spp.
  • Campylobacter spp.
  • Diarrhea
  • HIV
  • Helicobacter pylori
  • Intestinal inflammation
  • Lactoferrin
  • Venda

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