Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children

A. Chouikha; I. Fodha; M. Ben Hadj Fredj; M. Ardhaoui; N. Teleb; I. Brini; F. Messaadi; M. Mastouri; T. Sfar; M. Hachicha; T. Kammoun; A. Bouaaziz; F. Amri; A. Harbi; M. Zribi; S. Bousnina; A. Khemakhem; N. Boujaafar; A. Trabelsi; A. D. Steele

doi:10.1016/j.patbio.2009.04.008

Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children

A. Chouikha, I. Fodha, M. Ben Hadj Fredj, M. Ardhaoui, N. Teleb, I. Brini, F. Messaadi, M. Mastouri, T. Sfar, M. Hachicha, T. Kammoun, A. Bouaaziz, F. Amri, A. Harbi, M. Zribi, S. Bousnina, A. Khemakhem, N. Boujaafar, A. Trabelsi^*, A. D. Steele

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Background: Rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to detect a relationship between electropherotype pattern and molecular characteristics of the rotavirus strains. Material and methods: Were analyzed 278 rotavirus-positive specimens by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR. Pearson's correlation tests were used for statistical analysis. Results: Twelve different electropherotypes were visualized, eight with a long profile (186 cases) and four with a short one (87 cases). Concerning VP7 types, G2 viral strains were found to be predominant and were detected in 91 specimens (32.7%). Strains with G1, G3, G4, G8 and G9 specificities were detected in 62 (22.3%), 82 (29.5%), 13 (4.7%), two (0.7%) and seven cases (2.5%), respectively. The results of VP4 genotyping showed a predominance of P[8] genotype which comprised half of the strains identified (139 cases, 50%). VP4 P[4], P[6] and P[11] were found in 83 (29.9%), 31 (11.1%) and 11 (4.0%) specimens, respectively. A high rate of mixed strains was also found (1.8% mixed electropherotypes, 7.6% G-mixed and 5% P-mixed strains). Electropherotype pattern of rotavirus strains was significantly correlated with VP7 genotype (p=0.018) and with VP4 genotype specificities (p<0.001).

Original language	English
Pages (from-to)	e43-e48
Journal	Pathologie Biologie
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/j.patbio.2009.04.008
Publication status	Published - Jun 2011
Externally published	Yes

Keywords

Electropherotype
PAGE
Rotavirus
VP4 genotype
VP7 genotype

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10.1016/j.patbio.2009.04.008

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Chouikha, A., Fodha, I., Ben Hadj Fredj, M., Ardhaoui, M., Teleb, N., Brini, I., Messaadi, F., Mastouri, M., Sfar, T., Hachicha, M., Kammoun, T., Bouaaziz, A., Amri, F., Harbi, A., Zribi, M., Bousnina, S., Khemakhem, A., Boujaafar, N., Trabelsi, A., & Steele, A. D. (2011). Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children. Pathologie Biologie, 59(3), e43-e48. https://doi.org/10.1016/j.patbio.2009.04.008

@article{5f1b76d3019243099340bc3b775ab4e5,

title = "Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children",

abstract = "Background: Rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to detect a relationship between electropherotype pattern and molecular characteristics of the rotavirus strains. Material and methods: Were analyzed 278 rotavirus-positive specimens by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR. Pearson's correlation tests were used for statistical analysis. Results: Twelve different electropherotypes were visualized, eight with a long profile (186 cases) and four with a short one (87 cases). Concerning VP7 types, G2 viral strains were found to be predominant and were detected in 91 specimens (32.7%). Strains with G1, G3, G4, G8 and G9 specificities were detected in 62 (22.3%), 82 (29.5%), 13 (4.7%), two (0.7%) and seven cases (2.5%), respectively. The results of VP4 genotyping showed a predominance of P[8] genotype which comprised half of the strains identified (139 cases, 50%). VP4 P[4], P[6] and P[11] were found in 83 (29.9%), 31 (11.1%) and 11 (4.0%) specimens, respectively. A high rate of mixed strains was also found (1.8% mixed electropherotypes, 7.6% G-mixed and 5% P-mixed strains). Electropherotype pattern of rotavirus strains was significantly correlated with VP7 genotype (p=0.018) and with VP4 genotype specificities (p<0.001).",

keywords = "Electropherotype, PAGE, Rotavirus, VP4 genotype, VP7 genotype",

author = "A. Chouikha and I. Fodha and {Ben Hadj Fredj}, M. and M. Ardhaoui and N. Teleb and I. Brini and F. Messaadi and M. Mastouri and T. Sfar and M. Hachicha and T. Kammoun and A. Bouaaziz and F. Amri and A. Harbi and M. Zribi and S. Bousnina and A. Khemakhem and N. Boujaafar and A. Trabelsi and Steele, {A. D.}",

note = "Funding Information: This present study was supported by grant V27/181/167 from the World Health Organization (EMRO). The authors declare no conflict of interest. We thank the staff of the laboratories of Microbiology of Sahloul for their technical help and the nursing staff of the Paediatric Units of all hospitals included in Tunisian Rotavirus Network for assisting in sample collection.",

year = "2011",

month = jun,

doi = "10.1016/j.patbio.2009.04.008",

language = "English",

volume = "59",

pages = "e43--e48",

journal = "Pathologie Biologie",

issn = "0369-8114",

publisher = "Elsevier Masson",

number = "3",

}

Chouikha, A, Fodha, I, Ben Hadj Fredj, M, Ardhaoui, M, Teleb, N, Brini, I, Messaadi, F, Mastouri, M, Sfar, T, Hachicha, M, Kammoun, T, Bouaaziz, A, Amri, F, Harbi, A, Zribi, M, Bousnina, S, Khemakhem, A, Boujaafar, N, Trabelsi, A & Steele, AD 2011, 'Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children', Pathologie Biologie, vol. 59, no. 3, pp. e43-e48. https://doi.org/10.1016/j.patbio.2009.04.008

TY - JOUR

T1 - Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children

AU - Chouikha, A.

AU - Fodha, I.

AU - Ben Hadj Fredj, M.

AU - Ardhaoui, M.

AU - Teleb, N.

AU - Brini, I.

AU - Messaadi, F.

AU - Mastouri, M.

AU - Sfar, T.

AU - Hachicha, M.

AU - Kammoun, T.

AU - Bouaaziz, A.

AU - Amri, F.

AU - Harbi, A.

AU - Zribi, M.

AU - Bousnina, S.

AU - Khemakhem, A.

AU - Boujaafar, N.

AU - Trabelsi, A.

AU - Steele, A. D.

N1 - Funding Information: This present study was supported by grant V27/181/167 from the World Health Organization (EMRO). The authors declare no conflict of interest. We thank the staff of the laboratories of Microbiology of Sahloul for their technical help and the nursing staff of the Paediatric Units of all hospitals included in Tunisian Rotavirus Network for assisting in sample collection.

PY - 2011/6

Y1 - 2011/6

N2 - Background: Rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to detect a relationship between electropherotype pattern and molecular characteristics of the rotavirus strains. Material and methods: Were analyzed 278 rotavirus-positive specimens by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR. Pearson's correlation tests were used for statistical analysis. Results: Twelve different electropherotypes were visualized, eight with a long profile (186 cases) and four with a short one (87 cases). Concerning VP7 types, G2 viral strains were found to be predominant and were detected in 91 specimens (32.7%). Strains with G1, G3, G4, G8 and G9 specificities were detected in 62 (22.3%), 82 (29.5%), 13 (4.7%), two (0.7%) and seven cases (2.5%), respectively. The results of VP4 genotyping showed a predominance of P[8] genotype which comprised half of the strains identified (139 cases, 50%). VP4 P[4], P[6] and P[11] were found in 83 (29.9%), 31 (11.1%) and 11 (4.0%) specimens, respectively. A high rate of mixed strains was also found (1.8% mixed electropherotypes, 7.6% G-mixed and 5% P-mixed strains). Electropherotype pattern of rotavirus strains was significantly correlated with VP7 genotype (p=0.018) and with VP4 genotype specificities (p<0.001).

AB - Background: Rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. Analysis of mobility of the 11 segments of genomic RNA by polyacrylamide gel electrophoresis (PAGE) yields a pattern which is characteristic for a particular rotavirus isolate. The group A rotaviruses can be further characterized by analysis of VP7 and VP4 genes specificities, responsible for rotavirus classification into G and P genotypes, respectively. The aim of the present study was to detect a relationship between electropherotype pattern and molecular characteristics of the rotavirus strains. Material and methods: Were analyzed 278 rotavirus-positive specimens by PAGE and G/P-genotyped by multiplex semi-nested RT-PCR. Pearson's correlation tests were used for statistical analysis. Results: Twelve different electropherotypes were visualized, eight with a long profile (186 cases) and four with a short one (87 cases). Concerning VP7 types, G2 viral strains were found to be predominant and were detected in 91 specimens (32.7%). Strains with G1, G3, G4, G8 and G9 specificities were detected in 62 (22.3%), 82 (29.5%), 13 (4.7%), two (0.7%) and seven cases (2.5%), respectively. The results of VP4 genotyping showed a predominance of P[8] genotype which comprised half of the strains identified (139 cases, 50%). VP4 P[4], P[6] and P[11] were found in 83 (29.9%), 31 (11.1%) and 11 (4.0%) specimens, respectively. A high rate of mixed strains was also found (1.8% mixed electropherotypes, 7.6% G-mixed and 5% P-mixed strains). Electropherotype pattern of rotavirus strains was significantly correlated with VP7 genotype (p=0.018) and with VP4 genotype specificities (p<0.001).

KW - Electropherotype

KW - PAGE

KW - Rotavirus

KW - VP4 genotype

KW - VP7 genotype

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U2 - 10.1016/j.patbio.2009.04.008

DO - 10.1016/j.patbio.2009.04.008

M3 - Article

C2 - 19481882

AN - SCOPUS:79958720533

SN - 0369-8114

VL - 59

SP - e43-e48

JO - Pathologie Biologie

JF - Pathologie Biologie

IS - 3

ER -

Relationship between electropherotypes and VP7/VP4 genotypes of group A rotaviruses detected between 2000 and 2007 in Tunisian children

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