Relevance of commercial diagnostic tests to detection of enteric adenovirus infections in South Africa

P. L. Moore; A. D. Steele; J. J. Alexander

doi:10.1128/jcm.38.4.1661-1663.2000

Relevance of commercial diagnostic tests to detection of enteric adenovirus infections in South Africa

P. L. Moore^*, A. D. Steele, J. J. Alexander

^*Corresponding author for this work

Virological Pathology

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The prevalence of enteric adenoviruses detected by an in-house enzyme- linked immunosorbent assay (the RIVM-ELISA) ranged from 13 to 38%, and subgroup F adenoviruses comprised 86%. All subgroup F adenoviruses reacted with both RIVM anti-adenovirus type 40 (Ad40) and anti-adenovirus type 41 (Ad41) monoclonal antibodies but were not detected by Adenoclone Type 40/41 enzyme immunoassay (EIA). The correlation between the Biotrin EIA and RIVM- ELISA results was low (26%). Immunospecific tests suggest that a significant proportion of enteric adenoviruses, possibly comprising previously unidentified or emerging types, are not detected by commercial diagnostic tests in South Africa.

Original language	English
Pages (from-to)	1661-1663
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	38
Issue number	4
DOIs	https://doi.org/10.1128/jcm.38.4.1661-1663.2000
Publication status	Published - 2000

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10.1128/jcm.38.4.1661-1663.2000

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abstract = "The prevalence of enteric adenoviruses detected by an in-house enzyme- linked immunosorbent assay (the RIVM-ELISA) ranged from 13 to 38%, and subgroup F adenoviruses comprised 86%. All subgroup F adenoviruses reacted with both RIVM anti-adenovirus type 40 (Ad40) and anti-adenovirus type 41 (Ad41) monoclonal antibodies but were not detected by Adenoclone Type 40/41 enzyme immunoassay (EIA). The correlation between the Biotrin EIA and RIVM- ELISA results was low (26%). Immunospecific tests suggest that a significant proportion of enteric adenoviruses, possibly comprising previously unidentified or emerging types, are not detected by commercial diagnostic tests in South Africa.",

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AB - The prevalence of enteric adenoviruses detected by an in-house enzyme- linked immunosorbent assay (the RIVM-ELISA) ranged from 13 to 38%, and subgroup F adenoviruses comprised 86%. All subgroup F adenoviruses reacted with both RIVM anti-adenovirus type 40 (Ad40) and anti-adenovirus type 41 (Ad41) monoclonal antibodies but were not detected by Adenoclone Type 40/41 enzyme immunoassay (EIA). The correlation between the Biotrin EIA and RIVM- ELISA results was low (26%). Immunospecific tests suggest that a significant proportion of enteric adenoviruses, possibly comprising previously unidentified or emerging types, are not detected by commercial diagnostic tests in South Africa.

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Relevance of commercial diagnostic tests to detection of enteric adenovirus infections in South Africa

Abstract

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