TY - JOUR
T1 - Rotavirus Strain diversity in the centre coast of tunisia from 2000 through 2003
AU - Trabelsi, Abdelhalim
AU - Fodha, Imene
AU - Chouikha, Anissa
AU - Fredj, Mouna Ben Hadj
AU - Mastouri, Maha
AU - Abdelaziz, Ahmed Ben
AU - Sfar, Tahar
AU - Essoussi, Ahmed Sahloul
AU - Jaoua, Samir
AU - Steele, A. Duncan
N1 - Funding Information:
Financial support: World Health Organization (V27/181/113 and V27/181/136) and the South African Medical Research Council. Potential conflicts of interest: none reported. Supplement sponsorship: This article is part of a supplement entitled “Rotavirus Infection in Africa: Epidemiology, Burden of Disease, and Strain Diversity,” which was prepared as a project of the Rotavirus Vaccine Program, a partnership among PATH, the World Health Organization, and the US Centers for Disease Control and Prevention, and was funded in full or in part by the GAVI Alliance. Presented in part: 4th African Rotavirus Symposium, Mauritius, July 2008. Reprints or correspondence: Dr A. Duncan Steele, 1455 Leary Way NW, Seattle, WA 98107 ([email protected]).
PY - 2010/9/1
Y1 - 2010/9/1
N2 - An epidemiological survey investigating rotavirus infection in children was undertaken in the coastal region of Tunisia from January 2000 through September 2003. A total of 309 fecal specimens were screened by enzyme-linked immunosorbent assay and latex agglutination assay for the presence of group A rotavirus antigen. The detection rate was 26.2%. Rotavirus outbreaks showed a temperature-dependant pattern (Pp ) but no significant association with rainfall. Rotavirus strains isolated were analyzed by .026 RNA polyacrylamide gel electrophoresis and were characterized antigenically by monoclonal antibodies to the VP6 subgroup. Eight RNA electropherotypes were identified, with 3 long and 5 short different RNA profiles. Among VP6 typeable strains, all isolates with a long electrophoretic pattern carried the subgroup II specificity, whereas those with a short profile belonged to subgroup I. In total, 48 rotavirus-positive samples were analyzed for G and P typing by reverse-transcription polymerase chain reaction. A total of 8 different G and P combinations were found: G1P[8] (35.7%), G1P[6] (21.4%), G2P[4] (4.8%), G3P[4] (4.8%), G4P[6] (4.8%), G8P[8] (4.8%), G3P[8] (2.3%), and G4P[8] (2.3%). Mixed infections were detected in 19.1% of stool samples. The emergence in Tunisia of unconventional types, such as G8VP7 specificity, highlights the need for a continual survey of the uncommon strains in North Africa.
AB - An epidemiological survey investigating rotavirus infection in children was undertaken in the coastal region of Tunisia from January 2000 through September 2003. A total of 309 fecal specimens were screened by enzyme-linked immunosorbent assay and latex agglutination assay for the presence of group A rotavirus antigen. The detection rate was 26.2%. Rotavirus outbreaks showed a temperature-dependant pattern (Pp ) but no significant association with rainfall. Rotavirus strains isolated were analyzed by .026 RNA polyacrylamide gel electrophoresis and were characterized antigenically by monoclonal antibodies to the VP6 subgroup. Eight RNA electropherotypes were identified, with 3 long and 5 short different RNA profiles. Among VP6 typeable strains, all isolates with a long electrophoretic pattern carried the subgroup II specificity, whereas those with a short profile belonged to subgroup I. In total, 48 rotavirus-positive samples were analyzed for G and P typing by reverse-transcription polymerase chain reaction. A total of 8 different G and P combinations were found: G1P[8] (35.7%), G1P[6] (21.4%), G2P[4] (4.8%), G3P[4] (4.8%), G4P[6] (4.8%), G8P[8] (4.8%), G3P[8] (2.3%), and G4P[8] (2.3%). Mixed infections were detected in 19.1% of stool samples. The emergence in Tunisia of unconventional types, such as G8VP7 specificity, highlights the need for a continual survey of the uncommon strains in North Africa.
UR - http://www.scopus.com/inward/record.url?scp=77955693777&partnerID=8YFLogxK
U2 - 10.1086/653580
DO - 10.1086/653580
M3 - Article
C2 - 20684712
AN - SCOPUS:77955693777
SN - 0022-1899
VL - 202
SP - S252-S257
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - SUPPL. 1
ER -