The influence of trichoderma viride cellulase enzyme concentration on saccharification of waste paper materials

Khomotso M.P. Mokatse, M. Alfred Mogale, J. Pieter H. Van Wyk*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Enzymatic hydrolysis of lignocellulosic materials is one of the major steps in the commercialization process of converting cellulosic substrates into bio-products. The saccharification of lignocellulosic materials is usually achieved by a synergistic action of an enzyme mixture consisting of multiple cellulase enzymes such as endo-and exo-glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. During the enzymatic hydrolysis of the paper materials the process started with an initial fast rate of hydrolysis followed by a rapid decrease of the rate towards the end of hydrolysis. Obtained from this investigation showed a direct relationship between sugar concentration released and increasing enzyme concentration used during the saccharification process. Most paper materials showed maximum sugar production at an enzyme concentration of 20 mg/mL except filter paper that was maximally degraded at an enzyme concentration of 10 mg/mL, brown envelope paper at an enzyme concentration of 10 mg/mL producing a sugar concentration of 13.22 mg/mL and resulted in a percentage saccharification of 18%. Pick ‘n Pay paper yielded the lowest amount of sugar (5.8 mg/mL) when treated with a very high enzyme concentration of 30 mg/mL causing a percentage saccharification of 19%. Although most paper materials were maximally bio-degraded with the same cellulase concentration the ratio of enzyme concentration to mass of paper material degraded is unique for each paper material.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalOnLine Journal of Biological Sciences
Issue number4
Publication statusPublished - 2021


  • Enzyme Concentration
  • Saccharification
  • T. viride Cellulase
  • Waste Paper


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