Whole genome analysis of human rotaviruses reveals single gene reassortant rotavirus strains in zambia

Wairimu M. Maringa, Julia Simwaka, Peter N. Mwangi, Evans M. Mpabalwani, Jason M. Mwenda, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was ge-netically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix®. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix® vaccine introduction.

Original languageEnglish
Article number1872
JournalViruses
Volume13
Issue number9
DOIs
Publication statusPublished - Sep 2021

Keywords

  • Amino acid
  • Genotype constellation
  • Intergenogroup reassortment
  • Rotarix®
  • Rotavirus
  • Whole genome

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