Whole genome analysis of human rotaviruses reveals single gene reassortant rotavirus strains in zambia

Wairimu M. Maringa; Julia Simwaka; Peter N. Mwangi; Evans M. Mpabalwani; Jason M. Mwenda; M. Jeffrey Mphahlele; Mapaseka L. Seheri; Martin M. Nyaga

doi:10.3390/v13091872

Whole genome analysis of human rotaviruses reveals single gene reassortant rotavirus strains in zambia

Wairimu M. Maringa, Julia Simwaka, Peter N. Mwangi, Evans M. Mpabalwani, Jason M. Mwenda, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga^*

^*Corresponding author for this work

Virological Pathology

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was ge-netically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix®. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix® vaccine introduction.

Original language	English
Article number	1872
Journal	Viruses
Volume	13
Issue number	9
DOIs	https://doi.org/10.3390/v13091872
Publication status	Published - Sept 2021

Keywords

Amino acid
Genotype constellation
Intergenogroup reassortment
Rotarix®
Rotavirus
Whole genome

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v13091872

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title = "Whole genome analysis of human rotaviruses reveals single gene reassortant rotavirus strains in zambia",

abstract = "Rotarix{\textregistered} vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was ge-netically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix{\textregistered}. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix{\textregistered} vaccine introduction.",

keywords = "Amino acid, Genotype constellation, Intergenogroup reassortment, Rotarix{\textregistered}, Rotavirus, Whole genome",

author = "Maringa, {Wairimu M.} and Julia Simwaka and Mwangi, {Peter N.} and Mpabalwani, {Evans M.} and Mwenda, {Jason M.} and Mphahlele, {M. Jeffrey} and Seheri, {Mapaseka L.} and Nyaga, {Martin M.}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = sep,

doi = "10.3390/v13091872",

language = "English",

volume = "13",

journal = "Viruses",

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AU - Maringa, Wairimu M.

AU - Simwaka, Julia

AU - Mwangi, Peter N.

AU - Mpabalwani, Evans M.

AU - Mwenda, Jason M.

AU - Mphahlele, M. Jeffrey

AU - Seheri, Mapaseka L.

AU - Nyaga, Martin M.

PY - 2021/9

Y1 - 2021/9

N2 - Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was ge-netically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix®. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix® vaccine introduction.

AB - Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was ge-netically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix®. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix® vaccine introduction.

KW - Amino acid

KW - Genotype constellation

KW - Intergenogroup reassortment

KW - Rotarix®

KW - Rotavirus

KW - Whole genome

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U2 - 10.3390/v13091872

DO - 10.3390/v13091872

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AN - SCOPUS:85115380634

SN - 1999-4915

VL - 13

JO - Viruses

JF - Viruses

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M1 - 1872

ER -

Whole genome analysis of human rotaviruses reveals single gene reassortant rotavirus strains in zambia

Abstract

Keywords

UN SDGs

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